Singh Pankaj Kumar, Devanna Basavantraya N, Dubey Himanshu, Singh Prabhakar, Joshi Gaurav, Kumar Roshan
Department of Biotechnology, University Centre for Research & Development, Chandigarh University, Mohali, Punjab, India.
National Rice Research Institute (ICAR), Cuttack, India.
Front Genome Ed. 2024 Jun 11;6:1415244. doi: 10.3389/fgeed.2024.1415244. eCollection 2024.
Rice, a staple food for a significant portion of the global population, faces persistent threats from various pathogens and pests, necessitating the development of resilient crop varieties. Deployment of resistance genes in rice is the best practice to manage diseases and reduce environmental damage by reducing the application of agro-chemicals. Genome editing technologies, such as CRISPR-Cas, have revolutionized the field of molecular biology, offering precise and efficient tools for targeted modifications within the rice genome. This study delves into the application of these tools to engineer novel alleles of resistance genes in rice, aiming to enhance the plant's innate ability to combat evolving threats. By harnessing the power of genome editing, researchers can introduce tailored genetic modifications that bolster the plant's defense mechanisms without compromising its essential characteristics. In this study, we synthesize recent advancements in genome editing methodologies applicable to rice and discuss the ethical considerations and regulatory frameworks surrounding the creation of genetically modified crops. Additionally, it explores potential challenges and future prospects for deploying edited rice varieties in agricultural landscapes. In summary, this study highlights the promise of genome editing in reshaping the genetic landscape of rice to confront emerging challenges, contributing to global food security and sustainable agriculture practices.
水稻是全球很大一部分人口的主食，面临着来自各种病原体和害虫的持续威胁，因此需要培育具有抗性的作物品种。在水稻中部署抗性基因是防治病害和通过减少农用化学品的使用来降低环境破坏的最佳做法。基因组编辑技术，如CRISPR-Cas，已经彻底改变了分子生物学领域，为水稻基因组内的靶向修饰提供了精确而高效的工具。本研究深入探讨了这些工具在水稻中设计抗性基因新等位基因的应用，旨在增强植物对抗不断演变的威胁的固有能力。通过利用基因组编辑的力量，研究人员可以引入量身定制的基因修饰，增强植物的防御机制，同时不损害其基本特性。在本研究中，我们综合了适用于水稻的基因组编辑方法的最新进展，并讨论了围绕转基因作物创建的伦理考量和监管框架。此外，还探讨了在农业环境中部署经编辑水稻品种的潜在挑战和未来前景。总之，本研究突出了基因组编辑在重塑水稻遗传格局以应对新出现的挑战方面的前景，为全球粮食安全和可持续农业实践做出贡献。
