a Departamento de Biologia Celular e Molecular , Universidade Federal da Paraíba , João Pessoa , Brazil.
b Departamento de Biotecnologia , Universidade Federal da Paraíba , João Pessoa , Brazil.
Immunopharmacol Immunotoxicol. 2019 Feb;41(1):102-108. doi: 10.1080/08923973.2018.1555845. Epub 2019 Jan 9.
Pyocyanin is a typical virulence factor, a common Gram-negative rod responsible for a wide range of severe nosocomial infections. There is evidence indicating that pyocyanin has multiple biological activities, but little is known about anti-inflammatory properties. This study investigated pyocyanin effect on nitric oxide and cytokine production in lipopolysaccharide (LPS)-activated murine peritoneal macrophages. Macrophages were incubated in the presence and absence of pyocyanin (1, 5, 10, 50, and 100 µM) with and without LPS (1 µg/mL). Nitric oxide production was determined by Griess reagent and tumor necrosis factor (TNF)-α and interleukin (IL)-1β production was assessed by enzyme-linked immunosorbent assay. In addition, pyocyanin effects on zymosan A-induced peritonitis in mice were evaluated. Pyocyanin (5 and 10 µM) decreased nitric oxide, TNF-α, and IL-1β production independent of macrophage death. On the other hand, , pyocyanin (5 mg/kg) was not able to affect leukocyte migration into the site of inflammation. Thus, our findings suggest that pyocyanin exerts anti-inflammatory effects on murine peritoneal macrophages, downregulating nitric oxide, TNF-α, and IL-1β levels, which seems to be independent of cell migration. These effects may represent a mechanism of immune evasion; nevertheless more detailed studies should be performed to confirm this hypothesis.
绿脓菌素是一种典型的毒力因子，是一种常见的革兰氏阴性杆菌，可导致广泛的严重医院获得性感染。有证据表明绿脓菌素具有多种生物学活性，但对其抗炎特性知之甚少。本研究探讨了绿脓菌素对脂多糖（LPS）激活的鼠腹腔巨噬细胞中一氧化氮和细胞因子产生的影响。在存在和不存在 LPS（1 µg/mL）的情况下，用绿脓菌素（1、5、10、50 和 100 µM）孵育巨噬细胞。通过格里斯试剂测定一氧化氮的产生，通过酶联免疫吸附试验测定肿瘤坏死因子（TNF）-α和白细胞介素（IL）-1β的产生。此外，还评估了绿脓菌素对酵母聚糖 A 诱导的腹膜炎小鼠的影响。绿脓菌素（5 和 10 µM）可降低一氧化氮、TNF-α和 IL-1β的产生，而不影响巨噬细胞死亡。另一方面，绿脓菌素（5 mg/kg）不能影响白细胞向炎症部位迁移。因此，我们的研究结果表明，绿脓菌素对鼠腹腔巨噬细胞具有抗炎作用，下调一氧化氮、TNF-α和 IL-1β水平，这似乎与细胞迁移无关。这些作用可能代表一种免疫逃避机制；然而，应该进行更详细的研究来证实这一假设。
