Academy of Scientific and Innovative Research, CSIR-National Environmental Engineering Research Institute, Nagpur, 440020, India.
Biotechnol Prog. 2019 May;35(3):e2774. doi: 10.1002/btpr.2774. Epub 2019 Jan 23.
Present study used Aspergillus terreus strain C1 isolated from mangrove soil for itaconic acid (IA) production from potato starch waste. Fermentation parameters were optimized by classical one factor approach and statistical experimental designs, such as Plackett-Burman and response surface designs. Anionic deionization of potato waste was found to be a very effective, economic, and easy way of improving IA production. The increase in IA production by deionization was found to correlate with removal of phosphate. In our knowledge, this is the first report on application of deionization of potato waste to enhance IA production. Other parameters like inoculum development conditions, pH, presence of peptone and certain salts in the medium also significantly affected IA production. IA production by strain C1 increased 143-fold during optimization when compared with the starting condition. The optimized IA level (35.75 g/L) was very close to the maximum production predicted by RSM (38.88 g/L). Bench scale production of IA was further optimized in 3-L stirred tank reactor by varying parameters like agitation and aeration rate. The maximum IA production of 29.69 g/L was obtained under the agitation speed of 200 rpm and aeration rate of 0.25 vvm. To the best of our knowledge, it is the first report on IA production from potato starch waste at bioreactor level. © 2019 American Institute of Chemical Engineers Biotechnol. Prog., 35: e2774, 2019.
本研究从红树林土壤中分离出的土曲霉 C1 用于从马铃薯淀粉废物中生产衣康酸（IA）。通过经典的单因素法和统计实验设计，如 Plackett-Burman 和响应面设计，对发酵参数进行了优化。发现对马铃薯废物进行阴离子去离子化是一种非常有效、经济和简便的方法，可以提高 IA 的产量。去离子化对 IA 产量的提高与磷酸盐的去除有关。据我们所知，这是首次报道将马铃薯废物的去离子化应用于提高 IA 产量。其他参数，如接种物的开发条件、pH 值、培养基中存在的蛋白胨和某些盐，也对 IA 的生产有显著影响。与起始条件相比，在优化过程中，C1 菌株的 IA 产量增加了 143 倍。优化后的 IA 水平（35.75g/L）非常接近 RSM 预测的最大产量（38.88g/L）。通过改变搅拌和通气速率等参数，在 3-L 搅拌釜式反应器中进一步优化了 IA 的分批生产。在搅拌速度为 200rpm 和通气率为 0.25vvm 的条件下，IA 的最大产量为 29.69g/L。据我们所知，这是首次在生物反应器水平上报道从马铃薯淀粉废物生产 IA。©2019 美国化学工程师协会生物技术进展，35：e2774，2019。
