Department of Clinical and Military Laboratory Medicine, College of Medical Laboratory Science , Army Medical University , 30 Gaotanyan Street , Shapingba District, Chongqing 400038 , China.
ACS Appl Mater Interfaces. 2019 Jan 30;11(4):3745-3752. doi: 10.1021/acsami.8b20144. Epub 2019 Jan 22.
DNA methylation is a key factor in the pathogenesis of gene expression diseases or malignancies. Thus, it has become a significant biomarker for the diagnosis and prognosis of these diseases. In this paper, we designed an ultrasensitive and specific electrochemical biosensor for DNA methylation detection. The platform consisted of stem-loop-tetrahedron composite DNA probes anchoring at a Au nanoparticle-coated gold electrode, a restriction enzyme digestion of HpaII, and signal amplification procedures including electrodeposition of Au nanoparticles, hybridization chain reaction, and horseradish peroxidase enzymatic catalysis. Under optimal conditions, the design showed a broad dynamic range from 1 aM to 1 pM and a detection limit of about 0.93 aM. The approach also showed ideal specificity, repeatability, and stability. The recovery test demonstrated that the design is a promising platform for DNA methylation detection under clinical circumstances and could meet the need for cancer diagnosis.
DNA 甲基化是基因表达疾病或恶性肿瘤发病机制中的一个关键因素。因此，它已成为这些疾病诊断和预后的重要生物标志物。在本文中，我们设计了一种用于 DNA 甲基化检测的超灵敏和特异的电化学生物传感器。该平台由锚定在金纳米粒子涂覆的金电极上的茎环四面体复合 DNA 探针、限制性内切酶 HpaII 的酶切以及信号放大步骤组成，包括金纳米粒子的电沉积、杂交链式反应和辣根过氧化物酶酶催化。在最佳条件下，该设计表现出从 1 aM 到 1 pM 的宽动态范围和约 0.93 aM 的检测限。该方法还表现出理想的特异性、重复性和稳定性。回收试验表明，该设计是一种有前途的临床条件下 DNA 甲基化检测平台，可以满足癌症诊断的需求。
