Universidade Estadual Paulista (UNESP), Instituto de Ciência e Tecnologia, Departamento de Odontologia Restauradora, Grupo Acadêmico de Pesquisa Clínica, São José dos Campos, São Paulo, Brasil.
University of Iowa, College of Dentistry and Dental Clinics, Department of Endodontics, Iowa City, Iowa, USA.
J Appl Oral Sci. 2019 Jan 7;27:e20180111. doi: 10.1590/1678-7757-2018-0111.
Several anti-proteolytic dentin therapies are being exhaustively studied in an attempt to reduce dentin bond degradation and improve clinical performance and longevity of adhesive restorations. This study assessed the effect of epigallocatechin-3-gallate (EGCG) on long-term bond strength when incorporated into adhesives.
Adhesive systems were formulated with EGCG concentrations of 0 wt%: (no EGCG; control); 0.5 wt% EGCG; 1.0 wt% EGCG, and 1.5 wt% EGCG. Flexural strength (FS), modulus of elasticity (ME), modulus of resilience (MR), compressive strength (CS), degree of conversion (DC), polymerization shrinkage (PS), percentage of water sorption (%WS), percentage of water solubility (%WL) and cytotoxicity properties were tested. Dentin microtensile bond strength (µTBS) was evaluated after 24 h and again after 6 months of water storage. The adhesive interface was analyzed using scanning electron microscopy (SEM).
No significant differences were found among the groups in terms of FS, ME, MR, CS and PS. EGCG-doped adhesives increased the DC relative to the control group. EGCG concentrations of 1.0 wt% and 0.5 wt% decreased the WS of adhesives. WL decreased in all cases in which EGCG was added to adhesives, regardless of the concentration. EGCG concentrations of 1.0 wt% and 0.5 wt% reduced cytotoxicity. EGCG concentrations of 1.0 wt% and 0.5 wt% preserved µTBS after 6 months of storage, while 1.5 wt% EGCG significantly decreased µTBS. SEM: the integrity of the hybrid layer was maintained in the 0.5 wt% and 1.0 wt% EGCG groups.
EGCG concentrations of 1.0 wt% and 0.5 wt% showed better biological and mechanical performance, preserved bond strength and adhesive interface, and reduced cytotoxicity.
为了减少牙本质粘结降解，提高粘结修复体的临床效果和长期寿命，目前正在深入研究几种抗蛋白水解牙本质治疗方法。本研究评估了将表没食子儿茶素没食子酸酯（EGCG）加入粘结剂中对长期粘结强度的影响。
将 EGCG 浓度分别为 0wt%（无 EGCG；对照）、0.5wt%EGCG、1.0wt%EGCG 和 1.5wt%EGCG 的粘结剂配方进行了配制。测试了弯曲强度（FS）、弹性模量（ME）、弹性恢复率（MR）、抗压强度（CS）、转化率（DC）、聚合收缩（PS）、吸水率（WS）、溶解度（WL）和细胞毒性。在 24 小时和 6 个月水储存后评估牙本质微拉伸粘结强度（µTBS）。使用扫描电子显微镜（SEM）分析粘结界面。
在 FS、ME、MR、CS 和 PS 方面，各组之间没有显著差异。与对照组相比，EGCG 掺杂粘结剂的 DC 增加。EGCG 浓度为 1.0wt%和 0.5wt%时，降低了粘结剂的 WS。在加入 EGCG 的所有情况下，WL 均降低，而与浓度无关。EGCG 浓度为 1.0wt%和 0.5wt%降低了细胞毒性。EGCG 浓度为 1.0wt%和 0.5wt%保存了 6 个月储存后的 µTBS，而 1.5wt%EGCG 显著降低了 µTBS。SEM：在 0.5wt%和 1.0wt%EGCG 组中，混合层的完整性得以维持。
EGCG 浓度为 1.0wt%和 0.5wt%时表现出更好的生物和机械性能，保持粘结强度和粘结界面，降低细胞毒性。
