Department of Restorative Dentistry, Dental School, Federal University of Ceara, Rua Monsenhor Furtado S/N, Bairro Rodolfo Teófilo, Fortaleza, CE, 60430-325, Brazil.
Paulo Picanço School of Dentistry, Fortaleza, Brazil.
Odontology. 2019 Jan;107(1):23-28. doi: 10.1007/s10266-018-0367-0. Epub 2018 May 23.
The aim was to assess dentin adhesion and physicochemical properties of experimental etch-and-rinse and self-etch adhesives doped with epigallocatechin-3-gallate (EGCG), a well-known collagen crosslinker obtained from green tea (Camellia sinensis). Experimental adhesives were prepared without (0 wt%), with 0.1 or 0.5 wt% EGCG addition. Their degree of conversion was surveyed by FTIR, and bar-shaped specimens were tested to obtain flexural strength and modulus initially and after 1-week ethanol storage. Extracted human molars were prepared, bonded, and cut into resin-dentin sticks for microtensile bond strength test, which was conducted after 24 h or 6-month water storage. Statistical analyses were performed with two-way ANOVA and Tukey's test (p < 0.05). Degree of conversion outcomes depicted a significant polymerization reduction by the addition of EGCG in self-etch adhesive in both concentrations. However, only 0.1% reduced the conversion of etch-and-rinse adhesive. Flexural modulus and strength were significantly diminished (p < 0.05) by the addition of both concentrations of EGCG for the two model adhesives. Dentin-bond strength was reduced after aging with the addition of EGCG to self-etch adhesive. Nevertheless, 0.5% EGCG increased the bond strength of etch-and-rinse adhesive after aging, conversely to the significant reduction for EGCG-free control adhesive. In conclusion, EGCG at 0.5% provides optimal improvements on dentin bonding without altering final polymerization of a model etch-and-rinse adhesive.
目的是评估牙本质黏附性和物理化学性能的实验酸蚀-冲洗和自酸蚀黏合剂掺杂表没食子儿茶素没食子酸酯（EGCG），一种来自绿茶（Camellia sinensis）的已知胶原蛋白交联剂。实验黏合剂的制备没有（0wt%），添加 0.1 或 0.5wt%EGCG。通过傅里叶变换红外光谱法测量其转化率，并对条形试件进行测试，以获得初始和 1 周乙醇储存后的弯曲强度和模量。提取人磨牙，进行黏合，切成树脂-牙本质棒，进行微拉伸黏结强度测试，在 24 小时或 6 个月水储存后进行。采用双向方差分析和 Tukey 检验进行统计分析（p<0.05）。转化率结果显示，在两种浓度下，EGCG 的添加显著降低了自酸蚀黏合剂的聚合。然而，只有 0.1%的浓度降低了酸蚀-冲洗黏合剂的转化率。添加两种浓度的 EGCG 都会显著降低两种模型黏合剂的弯曲模量和强度（p<0.05）。添加 EGCG 会降低自酸蚀黏合剂老化后的牙本质黏结强度。然而，0.5%的 EGCG 增加了酸蚀-冲洗黏合剂老化后的黏结强度，而无 EGCG 对照黏合剂则显著降低。总之，0.5%的 EGCG 可在不改变模型酸蚀-冲洗黏合剂最终聚合的情况下，提供最佳的牙本质黏合改善效果。
