Yang Hongye, Guo Jingmei, Deng Donglai, Chen Zhiyong, Huang Cui
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
College of Stomatology, Guangxi Medical University, Nanning, China.
J Dent. 2016 Jan;44:44-9. doi: 10.1016/j.jdent.2015.12.001. Epub 2015 Dec 3.
To determine the effect of the combined use of epigallocatechin-3-gallate (EGCG) and ethanol-wet bonding (EWB) on resin-dentin bonds.
Sixty molars were sectioned, polished, and randomly divided into six groups (n=10) according to the following pretreatments: group 1, water-wet bonding (WWB); group 2, WWB with 0.02% (w/v) EGCG; group 3, WWB with 0.1% EGCG; group 4, EWB; group 5, EWB with 0.02% EGCG; and group 6, EWB with 0.1% EGCG. An etch-and-rinse adhesive was then used, followed by the resin composites building. The microtensile bond strength (MTBS), failure modes and interfacial nanoleakage were separately determined after 24h water storage or 10,000 runs of thermocycling.
Both pretreatment method (P<0.05) and thermocycling (P<0.05) significantly influenced bond strength and nanoleakage. Irrespective of thermocycling, the 0.02% EGCG/ethanol (group 5) pretreated adhesive-dentin interfaces showed higher MTBS than the control group (P<0.05). Nanoleakage expression of all groups increased after thermocycling (P<0.05) except group 5. Adhesive failure was the main fracture pattern in all groups.
This study showed that pretreatment with 0.02% EGCG/ethanol solutions can effectively improve immediate bond strength and bond stability of etch-and-rinse adhesives on dentin.
The adjunctive application of EGCG and EWB provides a new strategy for dentists to obtain the desired bond effectiveness during adhesive restoration in clinical practice.
确定表没食子儿茶素-3-没食子酸酯(EGCG)与乙醇湿粘结(EWB)联合使用对树脂-牙本质粘结的影响。
将60颗磨牙进行切片、抛光,并根据以下预处理方法随机分为六组(n = 10):第1组,水湿粘结(WWB);第2组,含0.02%(w/v)EGCG的WWB;第3组,含0.1%EGCG的WWB;第4组,EWB;第5组,含0.02%EGCG的EWB;第6组,含0.1%EGCG的EWB。然后使用酸蚀冲洗粘结剂,随后构建树脂复合材料。在水储存24小时或进行10000次热循环后,分别测定微拉伸粘结强度(MTBS)、失效模式和界面纳米渗漏。
预处理方法(P<0.05)和热循环(P<0.05)均显著影响粘结强度和纳米渗漏。无论是否进行热循环,经0.02%EGCG/乙醇预处理(第5组)的粘结剂-牙本质界面的MTBS均高于对照组(P<0.05)。除第5组外,所有组的纳米渗漏表达在热循环后均增加(P<0.05)。粘结剂失效是所有组的主要断裂模式。
本研究表明,用0.02%EGCG/乙醇溶液预处理可有效提高酸蚀冲洗粘结剂在牙本质上的即时粘结强度和粘结稳定性。
EGCG与EWB的辅助应用为牙医在临床实践中的粘结修复过程中获得理想的粘结效果提供了一种新策略。