基于金-银纳米粒子的双链 DNA 功能化用于 17β-雌二醇的超灵敏检测：表面增强拉曼光谱法。
Double strand DNA functionalized Au@Ag Nps for ultrasensitive detection of 17β-estradiol using surface-enhanced raman spectroscopy.
机构信息
School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, Guangzhou Higher Education Mega Center, Guangzhou 510006, China.
School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Food Refrigeration and Computerized Food Technology (FRCFT), Agriculture and Food Science Centre, University College Dublin, National University of Ireland, Belfield, Dublin 4, Ireland.
出版信息
Talanta. 2019 Apr 1;195:419-425. doi: 10.1016/j.talanta.2018.10.021. Epub 2018 Oct 13.
A detection method for 17β-estradiol (E2) using surface-enhanced Raman scattering (SERS)-based aptamer sensor was presented. Raman reporter molecule Cy3 labeled E2-aptamer and DNA functionalized gold-silver core-shell nanoparticles (Au@Ag CS NPs) offered SERS with high sensitivity and selectivity. Based on the fabricated double strand DNA-immobilized gold-silver core-shell nanoparticles (Au@Ag NPs), SERS signal intensity of Raman reporter changed with the number of Cy3-labeled aptamer attached to the core-shell nanoparticles due to the strong binding affinity between the aptamers and E2 with different concentrations. A wide linear range from 1.0 × 10 to 1.0 × 10 was obtained for the detection of E2, with a low detection limit of 2.75 fM. This proposed method showed highly sensitive and selective for detecting E2, and could be used to determine E2 in actual samples.
本文提出了一种基于表面增强拉曼散射（SERS）适配体传感器的 17β-雌二醇（E2）检测方法。拉曼报告分子 Cy3 标记的 E2-适配体和 DNA 功能化的金银核壳纳米粒子（Au@Ag CS NPs）为 SERS 提供了高灵敏度和选择性。基于制备的双链 DNA 固定化金银核壳纳米粒子（Au@Ag NPs），由于适配体与不同浓度的 E2 之间具有很强的结合亲和力，因此附着在核壳纳米粒子上的 Cy3 标记适配体的数量变化会导致拉曼报告分子的 SERS 信号强度发生变化。该方法对 E2 的检测范围很宽，从 1.0×10到 1.0×10，检测限低至 2.75 fM。该方法对 E2 的检测具有高灵敏度和选择性，可用于实际样品中 E2 的测定。
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