Zhao Lichun, Zhong Yute, Liang Jie, Gao Hongwei, Tang Nong
College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, 530200, China.
J Coll Physicians Surg Pak. 2019 Apr;29(4):392-394. doi: 10.29271/jcpsp.2019.04.392.
The aim of this study was to determine the effect of Astragalus polysaccharide (APS) on the expression of vascular endothelial growth factor (VEGF) and epidermal growth factor receptor (EGFR) in mice with Lewis transplantable lung cancer. It was an experimental study carried out from June 2017 to January 2018 at the College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, China. Forty male SPF-level C57BL/6J mice were selected and inoculated with Lewis lung cancer cell suspension in the right axilla of the mice to establish a lung-cancer mouse model. The mice were randomly divided into a model control group and APS groups with high, middle and low dosages, respectively, 10 in each group. After inoculation of Lewis tumor cell suspension for 2 days, mice in the model control group were injected intraperitoneally with 50 mL/kg of 0.9% sodium chloride solution, whereas, mice in the APS groups with high, middle and low dosages were intraperitoneally injected with APS at 100, 50, 25 mg/kg, respectively. The research results showed that APS can effectively inhibit the growth and metastasis of Lewis lung cancer in mice, improve immune organ function, inhibit the protein expression of VEGF and EGFR in tumor tissues, and have a concentration-effect relationships.
本研究旨在探讨黄芪多糖（APS）对Lewis移植性肺癌小鼠血管内皮生长因子（VEGF）和表皮生长因子受体（EGFR）表达的影响。本实验研究于2017年6月至2018年1月在中国南宁广西中医药大学药学院开展。选取40只雄性SPF级C57BL/6J小鼠，于小鼠右腋窝接种Lewis肺癌细胞悬液，建立肺癌小鼠模型。将小鼠随机分为模型对照组和高、中、低剂量APS组，每组10只。接种Lewis肿瘤细胞悬液2天后，模型对照组小鼠腹腔注射50 mL/kg的0.9%氯化钠溶液，而高、中、低剂量APS组小鼠分别腹腔注射100、50、25 mg/kg的APS。研究结果表明，APS可有效抑制Lewis肺癌在小鼠体内的生长和转移，改善免疫器官功能，抑制肿瘤组织中VEGF和EGFR的蛋白表达，且具有浓度效应关系。
