Worley Kathryn E, Chin Amanda S, Wan Leo Q
Department of Biomedical Engineering, Rensselaer Polytechnic Institute, 110 8th Street, Troy NY 12180, USA.
Department of Biological Sciences, Rensselaer Polytechnic Institute, 110 8th Street, Troy NY 12180, USA.
Stem Cells Int. 2018 Dec 2;2018:1848605. doi: 10.1155/2018/1848605. eCollection 2018.
Left-right symmetry breaking is a complex developmental process and an important part of embryonic axis development. As of yet, the biophysical mechanism behind LR asymmetry establishment remains elusive for the overall asymmetry of embryos as well as for the organ-specific asymmetry. Here, we demonstrate that inherent cellular chirality is observable in the cells of early embryonic stages using a 3D Matrigel bilayer system. Differentiation of human embryonic stem cells to three lineages corresponding to heart, intestine, and neural tissues demonstrates phenotype-specific inherent chiral biases, complementing the current knowledge regarding organ development. The existence of inherent cellular chirality early in development and its correlation with organ asymmetry implicate cell chirality as a possible regulator in LR symmetry breaking.
左右对称破缺是一个复杂的发育过程，也是胚胎轴发育的重要组成部分。迄今为止，对于胚胎的整体不对称以及器官特异性不对称，左右不对称建立背后的生物物理机制仍然难以捉摸。在这里，我们证明，使用三维基质胶双层系统，在早期胚胎阶段的细胞中可以观察到内在的细胞手性。人类胚胎干细胞分化为对应于心脏、肠道和神经组织的三个谱系，显示出表型特异性的内在手性偏向，补充了当前关于器官发育的知识。发育早期内在细胞手性的存在及其与器官不对称的相关性表明，细胞手性可能是左右对称破缺的一个调节因子。
