Derua Y A, Kahindi S C, Mosha F W, Kweka E J, Atieli H E, Zhou G, Lee M-C, Githeko A K, Yan G
Department of Parasitology and Entomology, Kilimanjaro Christian Medical University College, Tumaini University Makumira, Moshi, Tanzania.
Department of Research Programmes, National Institute for Medical Research, Amani Research Centre, Tanga, Tanzania.
Med Vet Entomol. 2019 Jun;33(2):220-227. doi: 10.1111/mve.12353. Epub 2019 Jan 9.
The microbial larvicides Bacillus thuringiensis var. israelensis (Bti) and Bacillus sphaericus (Bs) (Bacillales: Bacillaceae) are well known for their efficacy and safety in mosquito control. In order to assess their potential value in future mosquito control strategies in western Kenya, the current study tested the susceptibility of five populations of Anopheles gambiae complex mosquitoes (Diptera: Culicidae), collected from five diverse ecological sites in this area, to Bti and Bs under laboratory conditions. In each population, bioassays were conducted with eight concentrations of larvicide (Bti/Bs) in four replicates and were repeated on three separate days. Larval mortality was recorded at 24 h or 48 h after the application of larvicide and subjected to probit analysis. A total of 2400 An. gambiae complex larvae from each population were tested for their susceptibility to Bti and Bs. The mean (± standard error of the mean, SEM) lethal concentration values of Bti required to achieve 50% and 95% larval mortality (LC and LC ) across the five populations were 0.062 (± 0.005) mg/L and 0.797 (± 0.087) mg/L, respectively. Corresponding mean (± SEM) values for Bs were 0.058 (± 0.005) mg/L and 0.451 (± 0.053) mg/L, respectively. Statistical analysis indicated that the five populations of An. gambiae complex mosquitoes tested were fully susceptible to Bti and Bs, and there was no significant variation in susceptibility among the tested populations.
微生物杀幼虫剂苏云金芽孢杆菌以色列变种（Bti）和球形芽孢杆菌（Bs）（芽孢杆菌目：芽孢杆菌科）在控制蚊虫方面的有效性和安全性广为人知。为了评估它们在肯尼亚西部未来蚊虫控制策略中的潜在价值，本研究在实验室条件下测试了从该地区五个不同生态地点采集的五组冈比亚按蚊复合种群（双翅目：蚊科）对Bti和Bs的敏感性。在每组种群中，用八种浓度的杀幼虫剂（Bti/Bs）进行生物测定，重复四次，并在三个不同日期重复进行。在施用杀幼虫剂后24小时或48小时记录幼虫死亡率，并进行概率分析。对每组种群共2400只冈比亚按蚊复合种群幼虫进行了对Bti和Bs的敏感性测试。在这五组种群中，达到50%和95%幼虫死亡率（LC50和LC95）所需的Bti平均（±平均标准误差，SEM）致死浓度值分别为0.062（±0.005）mg/L和0.797（±0.087）mg/L。Bs的相应平均（±SEM）值分别为0.058（±0.005）mg/L和0.451（±0.053）mg/L。统计分析表明，所测试的五组冈比亚按蚊复合种群对Bti和Bs完全敏感，且测试种群之间的敏感性没有显著差异。
