Allen J D, Thoma J A
Carbohydr Res. 1978 Mar;61:377-85. doi: 10.1016/s0008-6215(00)84497-3.
The action of Aspergillus oryzae alpha amylase on reducing-end, and uniformly radiolabeled maltotriose through maltodecaose has been studied. The enzyme is found to hydrolyze more than a single glycosidic bond during enzyme-substrate encounters. The extent of this repetitive attack is quantitated.
研究了米曲霉α淀粉酶对还原端以及通过麦芽三糖至麦芽十糖的均匀放射性标记的麦芽寡糖的作用。发现该酶在酶与底物相遇时会水解不止一个糖苷键。对这种重复攻击的程度进行了定量。