Department of Orthopedics, The Third Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong 510630, P.R. China.
Department of Orthopedic Surgery, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510260, P.R. China.
Int J Mol Med. 2019 Mar;43(3):1513-1521. doi: 10.3892/ijmm.2018.4042. Epub 2018 Dec 31.
MicroRNAs (miRNAs) have emerged as pivotal regulators in various physiological and pathological processes at the post‑transcriptional level, and may serve important roles in osteogenic differentiation. However, their roles and functions are not fully understood. In the present study, miR‑223‑5p was identified as a modulator of osteoblastic differentiation in MC3T3‑E1 cells. Reverse transcription‑quantitative polymerase chain reaction and western blotting demonstrated that miR‑223‑5p was significantly upregulated in MC3T3‑E1 cells following the induction of osteoblast differentiation. Overexpression of miR‑223‑5p promoted osteogenic differentiation both in vitro and vivo. Expression of histone deacetylase 2 (HDAC2), which acts as a negative regulator of osteogenesis, was regulated by miR‑223‑5p. Collectively, the results of the present study revealed a novel miR‑223‑5p/HDAC2 axis that regulates osteoblast differentiation, and may serve as a potential target for enhancing bone formation in vivo.
微小 RNA（miRNAs）在后转录水平上作为各种生理和病理过程的关键调节因子出现，并且可能在成骨分化中发挥重要作用。然而，其作用和功能尚不完全清楚。在本研究中，miR-223-5p 被鉴定为 MC3T3-E1 细胞成骨分化的调节剂。逆转录-定量聚合酶链反应和 Western blot 分析表明，miR-223-5p 在诱导成骨分化后在 MC3T3-E1 细胞中显著上调。miR-223-5p 的过表达促进了体外和体内的成骨分化。组蛋白去乙酰化酶 2（HDAC2）作为成骨的负调控因子，其表达受 miR-223-5p 调控。综上所述，本研究揭示了一个新的 miR-223-5p/HDAC2 轴，该轴调节成骨细胞分化，可能成为增强体内骨形成的潜在靶点。
