Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
Mol Med Rep. 2019 Mar;19(3):1509-1520. doi: 10.3892/mmr.2019.9816. Epub 2019 Jan 3.
(‑)‑Epigallocatechin‑3‑gallate (EGCG), a major constituent of green tea, is a potential anticancer agent, but the molecular mechanisms of its effects are not well‑understood. The present study was conducted to examine the mechanism of EGCG in lung cancer cells. Alterations in long non‑coding RNAs (lncRNAs) and mRNAs were investigated in lung cancer cells treated with EGCG by lncRNA microarray analysis. Furthermore, the functions and signaling pathways regulated by EGCG were predicted by bioinformatics analysis. A total of 960 lncRNAs and 1,434 mRNAs were significantly altered following EGCG treatment. These lncRNAs were distributed across nearly all human chromosomes and the mRNAs were involved in the cell cycle and the mitotic cell cycle process. Through a combination of microarray and bioinformatics analysis, 20 mRNAs predicted to serve a key role in the EGCG regulation were identified, and certain regulatory networks involving EGCG‑regulated lncRNAs were predicted. In conclusion, EGCG affects the expression of various lncRNAs and mRNAs in the cells, therefore affecting cell functions. The results of the present study provide an insight into the mechanism of EGCG, which may be useful for therapeutic development.
（-）-表没食子儿茶素没食子酸酯（EGCG）是绿茶的主要成分，是一种潜在的抗癌剂，但它的作用机制尚不清楚。本研究旨在探讨 EGCG 在肺癌细胞中的作用机制。通过 lncRNA 微阵列分析，研究了 EGCG 处理的肺癌细胞中长链非编码 RNA（lncRNA）和 mRNA 的变化。此外，通过生物信息学分析预测了 EGCG 调节的功能和信号通路。EGCG 处理后，有 960 个 lncRNA 和 1434 个 mRNA 显著改变。这些 lncRNA 分布在几乎所有人类染色体上，mRNA 参与细胞周期和有丝分裂细胞周期过程。通过微阵列和生物信息学分析相结合，鉴定出 20 个预测在 EGCG 调节中起关键作用的 mRNA，并预测了涉及 EGCG 调节的 lncRNA 的某些调控网络。总之，EGCG 影响细胞中各种 lncRNA 和 mRNA 的表达，从而影响细胞功能。本研究结果为 EGCG 的作用机制提供了深入了解，可能对治疗开发有用。
