Tao Mengyuan, Zhou Haoxian, Luo Kaiwen, Lu Juan, Zhang Yueling, Wang Fan
Department of Biology and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, 515063, China.
Department of Biology and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, 515063, China.
Dev Comp Immunol. 2019 Apr;93:89-92. doi: 10.1016/j.dci.2019.01.003. Epub 2019 Jan 7.
For the past decades, quantitative serum proteomics in mammals have been widely applied in biomarker screening. Various serum protein removal methods have been developed to effectively sequester serum abundant proteins. However, few methods have been found for the removal of arthropod serum abundant proteins. Here, gel filtration chromatography and ultracentrifugation methods were applied to remove hemocyanin from Litopeaneus vannamei serum. When shrimps were challenged with white spot syndrome virus (WSSV), a total of 486 serum proteins were identified using mass spectrometry, and 18 upregulated WSSV responsive proteins were identified with isobaric tags for relative and absolute quantification (iTRAQ). These results provide an effective method to remove hemocyanin from shrimp serum. With this method some previously unidentified WSSV responsive serum proteins were revealed, which would give us a better insight into the response of crustaceans to WSSV infection.
在过去几十年中，哺乳动物血清蛋白质组定量分析已广泛应用于生物标志物筛选。人们开发了各种血清蛋白去除方法，以有效去除血清中的丰富蛋白。然而，很少有方法可用于去除节肢动物血清中的丰富蛋白。在此，采用凝胶过滤色谱法和超速离心法从凡纳滨对虾血清中去除血蓝蛋白。当对虾受到白斑综合征病毒（WSSV）攻击时，通过质谱鉴定出总共486种血清蛋白，并使用相对和绝对定量等压标签（iTRAQ）鉴定出18种上调的WSSV反应蛋白。这些结果提供了一种从虾血清中去除血蓝蛋白的有效方法。通过该方法揭示了一些先前未鉴定的WSSV反应血清蛋白，这将使我们更好地了解甲壳类动物对WSSV感染的反应。
