Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR China.
Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR China; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
Fish Shellfish Immunol. 2019 Oct;93:851-862. doi: 10.1016/j.fsi.2019.08.039. Epub 2019 Aug 17.
This study aimed to use isobaric tags (IBTs) to investigate the immune response of the hepatopancreas of Marsupenaeus japonicas infected with Vibrio parahaemolyticus or white spot syndrome virus (WSSV). Liquid chromatography-tandem mass spectrometry and protein sequencing identified 1005 proteins. Among them, 109 proteins were upregulated and 94 were downregulated after V. parahaemolyticus infection. After WSSV infection, 130 proteins were identified as differentially abundant, including 88 that were upregulated and 42 were downregulated. Fifty-four proteins were identified as differentially abundant after both V. parahaemolyticus and WSSV infection. A number of proteins related to cytoskeletal processes, including actin and myosin, and apoptosis-related proteins were upregulated in shrimp after V. parahaemolyticus and WSSV infection, indicating that phagocytosis and apoptosis may be involved in the response to in V. parahaemolyticus or WSSV infection. Quantitative real-time PCR was carried out to verify the reliability of the proteomic data. These data provide a basis to characterize the immunity-related processes of shrimp in response to infection with WSSV or V. parahaemolyticus.
本研究旨在使用同位素标签（IBTs）来研究感染副溶血弧菌或白斑综合征病毒（WSSV）的日本对虾肝胰腺的免疫反应。液相色谱-串联质谱和蛋白质测序鉴定出 1005 种蛋白质。其中，109 种蛋白质在副溶血弧菌感染后上调，94 种蛋白质下调。感染 WSSV 后，鉴定出 130 种差异丰度蛋白质，包括 88 种上调和 42 种下调。在副溶血弧菌和 WSSV 感染后，鉴定出 54 种差异丰度蛋白质。一些与细胞骨架过程相关的蛋白质，包括肌动蛋白和肌球蛋白，以及与凋亡相关的蛋白质，在虾中被副溶血弧菌和 WSSV 感染后上调，表明吞噬作用和凋亡可能参与了对副溶血弧菌或 WSSV 感染的反应。实时定量 PCR 用于验证蛋白质组数据的可靠性。这些数据为描述虾对 WSSV 或副溶血弧菌感染的免疫相关过程提供了依据。
