Marine College, Shandong University at Weihai, Weihai, Shandong 264209, China.
Fish Shellfish Immunol. 2010 Sep;29(3):480-6. doi: 10.1016/j.fsi.2010.05.009. Epub 2010 May 24.
White spot syndrome virus (WSSV) infects all shrimp species and is the greatest detriment to shrimp culture. To better understand the mechanism of molecular responses to WSSV infection in Chinese white shrimp Fenneropenaeus chinensis, two dimensional electrophoresis (2-DE) was used. Differentially expressed proteins in the hepatopancreas of control and WSSV-injected Chinese white shrimp between (6, 12 and 24 h post-injection) were screened. Quantitative intensity analysis and mass spectrometry revealed that 54 spots of 47 proteins were significantly up-regulated, including some immune-related proteins, such as Toll receptor precursor, Leu-rich repeat protein, peroxinectin and serine proteinase-like protein. Fourteen spots of 13 proteins, such as heat shock protein, ATP synthase sub-unit beta and thrombospondin, were down-regulated in WSSV-infected shrimps. Protein expression patterns of the infected shrimp were drastically altered by WSSV infection. Some of the altered proteins were further investigated at the mRNA level using semi-quantitative reverse transcript PCR. These data may provide some information about shrimp proteins that participate in the WSSV infection process.
白斑综合征病毒 (WSSV) 感染所有虾类，是对虾养殖的最大危害。为了更好地了解中国对虾（Fenneropenaeus chinensis）对 WSSV 感染的分子反应机制，采用二维电泳（2-DE）进行研究。在对照组和 WSSV 注射的中国对虾的肝胰腺中筛选出（注射后 6、12 和 24 小时）差异表达的蛋白质。定量强度分析和质谱分析显示，47 种蛋白质中有 54 个斑点显著上调，其中包括一些免疫相关蛋白，如 Toll 受体前体、富含亮氨酸重复蛋白、过氧化物酶和丝氨酸蛋白酶样蛋白。在 WSSV 感染的虾中，有 13 种蛋白质的 14 个斑点下调，如热休克蛋白、ATP 合酶亚基β和血栓素。WSSV 感染使感染虾的蛋白质表达模式发生了剧烈变化。使用半定量逆转录 PCR 进一步在 mRNA 水平上研究了一些改变的蛋白质。这些数据可能为参与 WSSV 感染过程的虾类蛋白质提供了一些信息。
