Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR China.
Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR China.
Fish Shellfish Immunol. 2019 Apr;87:360-370. doi: 10.1016/j.fsi.2019.01.008. Epub 2019 Jan 8.
The effects of oral administration of enrofloxacin (ENR) and San-Huang-San (SHS), singly or in combination, on the survival performance, disease resistance, and immunity of Litopenaeus vannamei were investigated. After challenge with an AHPND-causing strain of Vibrio parahaemolyticus (VP), shrimp were immediately fed a drug-free diet, diets containing only ENR (20 mg·kg) or SHS (500 mg·kg) or diets containing low-dose (10 mg·kg ENR + 250 mg ·kg SHS), medium-dose (20 mg·kg ENR + 500 mg ·kg SHS), and high-dose (40 mg·kg ENR + 1000 mg ·kg SHS) drug combinations for 5 days. The cumulative shrimp mortality over 5 days after injection of VP in the ENR + SHS combination groups was significantly lower than that in the ENR or SHS alone groups (p < 0.05). Immune parameters, including the vibrio density, total hemocyte counts (THCs), hemocyanin (HEM) concentration, antibacterial activity, activity levels of lysozyme (LZM), acid phosphatase (ACP), alkaline phosphatase (AKP), and phenoloxidase (PO) in cell-free hemolymph, and the expression levels of the immune-related genes anti-lipopolysaccharide factor (ALF), cathepsin B (catB), crustin, lectin (Lec), lysozyme (LZM), and Toll-like receptor (TLR) in hemocytes were determined in the shrimp. The results showed that the shrimp in drug combination groups cleared more VP than that in the ENR or SHS group in the same time. The values for other immune parameters in the drug combination groups were higher than those in the ENR or SHS group (p < 0.05). Finally, in the histological examinations, the histological structural alignment and integrity of the hepatopancreatic tubules in the drug combination groups were better than that in the ENR and SHS groups. Under the experimental conditions, compared with ENR or SHS used alone, the combination use of ENR and SHS could improve immunity and disease resistance in shrimp after VP infection, and could reduce the use of ENR when the better therapeutic effect was achieved.
本研究旨在探讨恩诺沙星（ENR）和三黄散（SHS）单独或联合口服给药对凡纳滨对虾生存性能、抗病力和免疫力的影响。在感染 AHPND 相关副溶血弧菌（VP）后，对虾立即投喂无药饲料，饲料中仅含 ENR（20mg·kg）或 SHS（500mg·kg）或低剂量（10mg·kg ENR+250mg·kg SHS）、中剂量（20mg·kg ENR+500mg·kg SHS）和高剂量（40mg·kg ENR+1000mg·kg SHS）药物组合，连续投喂 5 天。与 ENR 或 SHS 单独给药组相比，ENR+SHS 联合给药组在注射 VP 后 5 天内的累计死亡率显著降低（p<0.05）。在无药饲料中添加 ENR 和 SHS 可提高对虾的免疫参数，包括副溶血弧菌密度、总血细胞计数（THC）、血蓝蛋白（HEM）浓度、抗菌活性、细胞游离血淋巴中溶菌酶（LZM）、酸性磷酸酶（ACP）、碱性磷酸酶（AKP）和酚氧化酶（PO）的活力，以及血细胞中免疫相关基因抗脂多糖因子（ALF）、组织蛋白酶 B（catB）、壳质、凝集素（Lec）、溶菌酶（LZM）和 Toll 样受体（TLR）的表达水平。结果表明，在相同时间内，药物联合组清除的 VP 多于 ENR 或 SHS 组。药物联合组的其他免疫参数值均高于 ENR 或 SHS 组（p<0.05）。最后，在组织学检查中，药物联合组的肝胰腺小管组织结构排列和完整性均优于 ENR 和 SHS 组。在实验条件下，与单独使用 ENR 或 SHS 相比，ENR 和 SHS 联合使用可提高 VP 感染后虾的免疫力和抗病力，且在达到更好的治疗效果时可减少 ENR 的使用量。
