Kim Hyeon-Cheol, Choe Changyoung, Kim SuHee, Chae Joon-Seok, Yu Do-Hyeon, Park Jinho, Park Bae-Keun, Choi Kyoung-Seong
College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Korea.
Animal Disease & Biosecurity Team, National Institute of Animal science, Rural Development Administration, Wanju 55365, Korea.
Korean J Parasitol. 2018 Dec;56(6):619-623. doi: 10.3347/kjp.2018.56.6.619. Epub 2018 Dec 31.
Bovine coccidiosis is one of the most important parasitic diseases affecting calf productivity. Here, we investi- gated the prevalence of Eimeria spp. in pre-weaned native Korean calves and determined the correlation between diar- rhea and Eimeria spp. Fecal samples were collected from individual calves (288 normal and 191 diarrheic) in 6 different farms. Of the 479 samples, Eimeria oocysts were detected in 124 calves (25.9%). Five Eimeria spp. were identified; E. zuernii (18.8%) was the most prevalent, followed by E. auburnensis (12.5%), E. bovis (7.5%), E. subspherica (5.8%), and E. bukidnonensis (1.0%). A significant correlation was observed between diarrhea and mixed infection with more than 2 Eimeria spp. (odds ratio [OR]=2.21; 95% confidence interval [CI]: 1.09-4.49; P=0.03) compared to single infection (OR = 1.29; 95% CI: 0.77-2.15; P = 0.33). Of the 5 Eimeria spp. identified, E. subspherica (95% CI: 1.24-5.61; P = 0.01) and E. bukidnonensis (95% CI: 825.08-1,134.25; P = 0.00) strongly increased the risk of diarrhea by 2.64-fold and 967.39-fold, respectively, compared to other species. Moreover, mixed infection with E. auburnensis and E. bukidnonensis was significantly associated with diarrhea (OR = 2,388.48; 95% CI: 1,009.71-5,650.00; P < 0.00) in pre-weaned native Korean calves. This is the first report to demonstrate the importance of E. bukidnonensis associated with diarrhea in pre-weaned native Korean calves. Further epidemiological studies should investigate the prevalence of E. bukidnonensis and the association between E. bukidnonensis and diarrhea.
牛球虫病是影响犊牛生产性能的最重要的寄生虫病之一。在此，我们调查了韩国本地断奶前犊牛艾美耳球虫属的感染率，并确定了腹泻与艾美耳球虫属之间的相关性。从6个不同农场的个体犊牛（288头正常犊牛和191头腹泻犊牛）采集粪便样本。在479份样本中，124头犊牛（25.9%）检测到艾美耳球虫卵囊。鉴定出5种艾美耳球虫；最常见的是zuernii艾美耳球虫（18.8%），其次是奥伯恩艾美耳球虫（12.5%）、牛艾美耳球虫（7.5%）、球形艾美耳球虫（5.8%）和布基农艾美耳球虫（1.0%）。与单一感染（比值比[OR]=1.29；95%置信区间[CI]：0.77 - 2.15；P = 0.33）相比，腹泻与2种以上艾美耳球虫混合感染之间存在显著相关性（OR = 2.21；95% CI：1.09 - 4.49；P = 0.03）。在鉴定出的5种艾美耳球虫中，球形艾美耳球虫（95% CI：1.24 - 5.61；P = 0.01）和布基农艾美耳球虫（95% CI：825.08 - 1,134.25；P = 0.00）分别使腹泻风险显著增加2.64倍和967.39倍，相比其他种类。此外，在韩国本地断奶前犊牛中，奥伯恩艾美耳球虫和布基农艾美耳球虫混合感染与腹泻显著相关（OR = 2388.48；95% CI：1009.71 - 5650.00；P < 0.00）。这是首次报道证明布基农艾美耳球虫与韩国本地断奶前犊牛腹泻相关的重要性。进一步的流行病学研究应调查布基农艾美耳球虫的感染率以及布基农艾美耳球虫与腹泻之间的关联。
