MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Crewe Rd, Edinburgh EH4 2XU, UK.
MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Crewe Rd, Edinburgh EH4 2XU, UK.
Trends Cell Biol. 2019 Mar;29(3):201-211. doi: 10.1016/j.tcb.2018.12.003. Epub 2019 Jan 7.
A significant amount of RNA is present in the nucleus of mammalian cells but only a small proportion of it is destined for the cytoplasm and subsequent translation, leaving much RNA to associate with chromatin. Historically, nuclear RNA was thought to interact with proteins to form a filamentous nuclear matrix, but this idea became less popular as more dynamic models of chromatin behaviour became more prevalent. Using new molecular and imaging approaches it is becoming clear that RNA should be considered an integral component of nuclear organisation; it is transcriptionally responsive and interacts with abundant nuclear RNA-binding proteins. We suggest that these protein/RNA structures form a dynamic nuclear mesh that can regulate interphase chromatin structure.
大量的 RNA 存在于哺乳动物细胞的核内，但只有一小部分 RNA 注定要进入细胞质并进行后续翻译，其余的 RNA 与染色质结合。历史上，核内 RNA 被认为与蛋白质相互作用形成丝状核基质，但随着更多动态的染色质行为模型变得越来越流行，这种观点变得不那么流行了。使用新的分子和成像方法，越来越清楚的是，RNA 应该被视为核组织的一个组成部分；它对转录有反应，并与丰富的核 RNA 结合蛋白相互作用。我们认为，这些蛋白/RNA 结构形成了一个动态的核网格，可以调节间期染色质结构。
