Muangkaew Watcharamat, Suwanmanee San, Singkum Pantira, Pumeesat Potjaman, Luplertlop Natthanej
Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Interdiscip Perspect Infect Dis. 2018 Dec 2;2018:3748594. doi: 10.1155/2018/3748594. eCollection 2018.
and are important fungal species isolated from immunocompromised patients. Previous studies have demonstrated that these filamentous fungi exist as saprophytes in the soil and showed the highest minimum inhibitory concentration to several drugs. We aimed to examine how UVC affects the and by investigating the role of UVC on growth, induction of apoptosis by ethidium bromide (EB)/acridine orange (AO) staining, and transcriptomic study of caspase recruitment domain family, member 9 (CARD-9) gene. Our studies showed that 15 minutes of exposure to UVC light effectively increased reduction in both organisms and caused changes in colony morphology, color, and hyphal growth pattern. After 15 min of UVC irradiation, apoptotic cells were quantitated by EB/AO staining, and the percentage of apoptosis was 96.06% in and 28.30% in . CARD-9 gene expression results confirmed that apoptosis was induced in and after UVC treatment and that showed a higher expression of apoptosis signaling than . Our study explored the effects of UVC in the inactivation of and . We hope that our data is useful to other researchers in future studies.
[未提及具体菌名]和[未提及具体菌名]是从免疫功能低下患者中分离出的重要真菌物种。先前的研究表明，这些丝状真菌在土壤中以腐生菌形式存在，并且对几种药物表现出最高的最低抑菌浓度。我们旨在通过研究紫外线（UVC）对生长的作用、通过溴化乙锭（EB）/吖啶橙（AO）染色诱导细胞凋亡以及胱天蛋白酶募集结构域家族成员9（CARD-9）基因的转录组学研究，来探讨UVC如何影响[未提及具体菌名]和[未提及具体菌名]。我们的研究表明，暴露于UVC光15分钟可有效增加两种生物体的减少，并导致菌落形态、颜色和菌丝生长模式发生变化。在UVC照射15分钟后，通过EB/AO染色对凋亡细胞进行定量，[未提及具体菌名]中的凋亡百分比为96.06%，[未提及具体菌名]中的凋亡百分比为28.30%。CARD-9基因表达结果证实，UVC处理后[未提及具体菌名]和[未提及具体菌名]中均诱导了细胞凋亡，并且[未提及具体菌名]显示出比[未提及具体菌名]更高的凋亡信号表达。我们的研究探索了UVC在[未提及具体菌名]和[未提及具体菌名]灭活中的作用。我们希望我们的数据对未来其他研究人员有用。
