Department of Occupational Health, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Biostatistics and Epidemiology, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Environ Sci Pollut Res Int. 2019 Mar;26(7):6868-6876. doi: 10.1007/s11356-019-04179-1. Epub 2019 Jan 11.
To evaluate the potential applicability of carbon load in airway macrophages as a marker of exposure to traffic-related air pollution (TRAP) and its association with parameters of comet assay as a marker of DNA damage, and pulmonary function tests (PFTs) in the group of taxi drivers in Iran. One hundred four male taxi drivers with at least 1-year job history were randomly selected from registered drivers in the taxi union. Airway macrophages were obtained via sputum induction, and then the area of airway macrophages occupied by carbon was measured. DNA damage was determined by comet assay. PFTs were measured by the spirometer. Most of the participants (89.4%) were non-smoker. In this study, 52.7% of non-smoker participants were able to give a sample of sputum with macrophage. Carbon content of airway macrophages was 0.2 μm. There was no significant difference in pulmonary function and comet assay parameters in terms of smoking status. There was an inverse correlation between carbon load with each of comet assay and PFTs parameters, although not statistically significant. This study identified that long-term exposure to TRAP can be a risk factor for pulmonary disorders.
为了评估气道巨噬细胞中的碳负荷作为暴露于交通相关空气污染 (TRAP) 的标志物的潜在适用性，以及其与彗星试验作为 DNA 损伤标志物的参数之间的关联，并评估肺功能测试 (PFT) 在伊朗出租车司机群体中的作用。从出租车工会注册的司机中随机选择了 104 名至少有 1 年工作经验的男性出租车司机。通过痰液诱导获得气道巨噬细胞，然后测量气道巨噬细胞中被碳占据的区域。通过彗星试验测定 DNA 损伤。使用肺活量计测量 PFTs。大多数参与者（89.4%）为非吸烟者。在这项研究中，52.7%的非吸烟者能够提供含有巨噬细胞的痰液样本。气道巨噬细胞中的碳含量为 0.2μm。吸烟状况与肺功能和彗星试验参数之间无显著差异。碳负荷与彗星试验和 PFTs 参数之间存在负相关关系，但无统计学意义。这项研究表明，长期暴露于 TRAP 可能是肺部疾病的一个风险因素。
