Youjiang Medical University for Nationalities, Baise, China.
Reproductive Medicine Center, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China.
J Cell Mol Med. 2019 Apr;23(4):2410-2418. doi: 10.1111/jcmm.14067. Epub 2019 Jan 10.
Interleukin-27 (IL-27) gene polymorphisms are linked to infectious disease susceptibility and IL-27 plasma level is associated with HIV infection. Therefore, we aimed to investigate the association between IL-27 polymorphisms and susceptibility to HIV infection and disease progression. A total of 300 patients with HIV infection (48 long-term nonprogressors and 252 typical progressors) and 300 healthy controls were genotyped for three IL-27 polymorphisms, rs17855750, rs181206, rs40837 which were performed by using multiple single nucleotide primer extension technique. Significant association was found between IL-27 rs40837 polymorphisms with susceptibility to HIV infection (AG vs AA: adjusted OR = 1.60, 95% CI, 1.11-2.30, P = 0.012; AG+GG vs AA: adjusted OR = 1.44, 95% CI, 1.02-2.03, P = 0.038) and disease progression (LTNP: AG vs AA: adjusted OR = 2.33, 95% CI, 1.13-4.80, P = 0.021; TP: AG vs AA: adjusted OR = 1.50, 95% CI, 1.04-2.24, P = 0.030). Serum IL-27 levels were significantly lower in cases compared to controls (P < 0.001). There were lower serum IL-27 levels in TPs than in LTNPs (P < 0.001). We further found that LTNPs with rs40837 AG or GG genotype had lower serum IL-27 levels than with AA genotype (P < 0.05). The CD4 T counts in cases were significantly lower than controls (P < 0.001). In contrast, individuals with rs40837 AG genotype had lower CD4 T counts than with AA genotype in cases (P < 0.05). In addition, CD4 T counts in TPs were significantly lower than LTNPs (P < 0.001). IL-27 rs40837 polymorphism might influence the susceptibility to HIV infection and disease progression probably by regulating the level of serum IL-27 or the quantity of CD4 T.
白细胞介素-27 (IL-27) 基因多态性与传染病易感性有关，IL-27 血浆水平与 HIV 感染有关。因此，我们旨在研究 IL-27 多态性与 HIV 感染易感性和疾病进展的关系。共对 300 例 HIV 感染者（48 例长期非进展者和 252 例典型进展者）和 300 名健康对照者进行了 IL-27 三种多态性(rs17855750、rs181206、rs40837)的基因分型，采用多重单核苷酸引物延伸技术进行检测。结果发现，IL-27 rs40837 多态性与 HIV 感染易感性有关（AG 与 AA：调整后的 OR=1.60，95%CI，1.11-2.30，P=0.012；AG+GG 与 AA：调整后的 OR=1.44，95%CI，1.02-2.03，P=0.038）和疾病进展（LTNP：AG 与 AA：调整后的 OR=2.33，95%CI，1.13-4.80，P=0.021；TP：AG 与 AA：调整后的 OR=1.50，95%CI，1.04-2.24，P=0.030）。与对照组相比，病例组血清 IL-27 水平显著降低（P<0.001）。与 LTNPs 相比，TPs 中的血清 IL-27 水平较低（P<0.001）。我们还发现，与 AA 基因型相比，rs40837 AG 或 GG 基因型的 LTNPs 血清 IL-27 水平较低（P<0.05）。病例组的 CD4 T 计数明显低于对照组（P<0.001）。相反，与 AA 基因型相比，病例中 rs40837 AG 基因型的 CD4 T 计数较低（P<0.05）。此外，TPs 中的 CD4 T 计数明显低于 LTNPs（P<0.001）。IL-27 rs40837 多态性可能通过调节血清 IL-27 水平或 CD4 T 细胞数量来影响 HIV 感染易感性和疾病进展。
