Abbasi Sumaira, Khattak Saima Mumtaz, Malik Sana
Department of Anatomy, Federal Medical and Dental College, Islamabad, Pakistan.
J Ayub Med Coll Abbottabad. 2018 Oct-Dec;30(4):592-595.
This study was conducted to evaluate the effect of Vitamin C on Monosodium Glutamate induced histopathological changes in oviduct of adult female Sprague Dawley rats. The duration of study was 6 months. It was an analytical experimental randomized control trial.
In this experimental study, 45 female adult Sprague Dawley rats of 10-14 weeks were used and divided into 3 groups. Each group contains 15 rats. Control group (C) received standard laboratory diet. Experimental group A, was given Monosodium Glutamate (0.08 mg/kg body weight/ day) whereas experimental group B, was served on both MSG and Vitamin C (250 mg/kg body weight/day). All groups received diet for a period of 4 weeks. After 4 weeks all rats were sacrificed and oviducts were obtained. For the study of tissue under light microscopy, tissue processing was done by using Haematoxylin and Eosin stain and 5micrometer thick sections were taken from the ampullary part of oviduct.
After administration of MSG, group A showed vacuolization of epithelial cells, infiltration of RBCs in lumen with substantial decrease in the diameter of oviduct in group A. Protective effects were seen in vitamin C supplemented group B, with decrease in epithelial vacuolization and RBCs infiltrate along with increase in diameter of oviduct.
Vitamin C has protective effect on Monosodium Glutamate induced histological changes in oviduct of rats.
本研究旨在评估维生素C对成年雌性斯普拉格-道利大鼠输卵管中味精诱导的组织病理学变化的影响。研究持续时间为6个月。这是一项分析性实验随机对照试验。
在本实验研究中，使用了45只10 - 14周龄的成年雌性斯普拉格-道利大鼠，并将其分为3组。每组包含15只大鼠。对照组（C）给予标准实验室饮食。实验组A给予味精（0.08毫克/千克体重/天），而实验组B给予味精和维生素C（250毫克/千克体重/天）。所有组均喂食4周。4周后，处死所有大鼠并获取输卵管。为了在光学显微镜下研究组织，使用苏木精和伊红染色进行组织处理，并从输卵管壶腹部取5微米厚的切片。
给予味精后，A组显示上皮细胞空泡化，管腔内有红细胞浸润，且A组输卵管直径大幅减小。在补充维生素C的B组中观察到保护作用，上皮空泡化减少，红细胞浸润减少，同时输卵管直径增加。
维生素C对味精诱导的大鼠输卵管组织学变化具有保护作用。
