El-Meghawry El-Kenawy Ayman, Osman Hosam Eldin Hussein, Daghestani Maha Hasan
Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, Minufiya University, Egypt.
Exp Toxicol Pathol. 2013 Jul;65(5):513-21. doi: 10.1016/j.etp.2012.02.007. Epub 2012 Jun 2.
Monosodium glutamate (MSG) is a commonly used food enhancer. Glutamate is used as food additive for enhancing the "meat flavor" of food and gives a particular taste named "umami". In this study, we evaluated the effect of vitamin C on monosodium glutamate induced rat liver injury. This study was divided into 3 groups: group 1 received a diet containing 0.9% NaCl; group 2 received diet containing MSG 6 mg/g/b.w.; and group 3 received a diet containing 6 mg/g/b.w. followed by vitamin C (500 mg/kg/b.w.) for 45 days. The resulting changes were detected using histological, histochemical, ultrastructural, and immuohistochemical analysis. Severe alterations were recorded including dilatations of the central veins; severe cyto-architectural distortions of the hepatocytes; marked reduction in both carbohydrates and proteins; vacuolated cytoplasm, swollen mitochondria and vesiculated rough endoplasmic reticulum with picknotic nuclei; in addition to significant variation in the expression of ki-67 and p53 proteins. The data obtained from this study showed the improvements in the pathological architecture of the liver after treatment with vitamin C. The present data point to the ameliorative effect of vitamin C against MSG induced liver injury.
味精（MSG）是一种常用的食品增味剂。谷氨酸被用作食品添加剂以增强食物的“肉香味”，并赋予一种名为“鲜味”的特殊味道。在本研究中，我们评估了维生素C对味精诱导的大鼠肝损伤的影响。本研究分为3组：第1组给予含0.9%氯化钠的饮食；第2组给予含6毫克/克/体重味精的饮食；第3组给予含6毫克/克/体重味精的饮食，随后给予维生素C（500毫克/千克/体重），持续45天。使用组织学、组织化学、超微结构和免疫组织化学分析检测所产生的变化。记录到严重改变，包括中央静脉扩张；肝细胞严重的细胞结构扭曲；碳水化合物和蛋白质均显著减少；细胞质空泡化、线粒体肿胀、糙面内质网呈泡状且细胞核固缩；此外，ki-67和p53蛋白的表达有显著变化。本研究获得的数据显示，用维生素C治疗后肝脏的病理结构有所改善。目前的数据表明维生素C对味精诱导的肝损伤具有改善作用。
