Philipsen E K, Clausen H, Dabelsteen E, Graem N
Department of Medicine F, Glostrup Hospital, Denmark.
APMIS. 1988 Dec;96(12):1109-17. doi: 10.1111/j.1699-0463.1988.tb00988.x.
Many tumor-associated carbohydrate antigens are related to the blood group systems. Since several of these antigens are developmentally regulated, a systematic knowledge of the expression of blood group related carbohydrate antigens during organogenesis is important. By immunohistochemical methods we investigated the expression of carbohydrate structures related to the ABH, Lewis, T and Tn blood group systems in 28 fetal pancreas, from 13th-40th gestational week using a comprehensive set of well-defined monoclonal antibodies, reacting with type 1, 2, 3, and 4 chain carbohydrate structures. The following antigens were found in fetal pancreas: Type 1 chain: Lea, Leb, monosialylated Lea and disialylated Lea, type 2 chain: N-acetyllactosamine (the immediate precursor to blood group H antigen), branched N-acetyllactosamine, H-antigen, Lex and Ley; type 3 chain: H-antigen. The T-antigen was well expressed, whereas this was not the case with the Tn-antigen. As expected the A-antigen was found in 10 of 24 cases. The A-related antigens: ALeb, ALed and ALey were only found in a few of these ten specimens whereas type 3 chain A-repetitive was found in all of them. Since msLea, Lex, Ley and T-antigen have been described as tumor-associated antigens, we conclude that several carbohydrate tumorrelated antigens are expressed in fetal pancreas.
许多肿瘤相关碳水化合物抗原与血型系统相关。由于其中一些抗原受发育调控，因此系统了解器官发生过程中血型相关碳水化合物抗原的表达情况很重要。我们采用免疫组织化学方法，使用一组全面且定义明确的单克隆抗体，这些抗体可与1型、2型、3型和4型链碳水化合物结构发生反应，研究了28例孕13至40周胎儿胰腺中与ABH、Lewis、T和Tn血型系统相关的碳水化合物结构的表达。在胎儿胰腺中发现了以下抗原：1型链：Lea、Leb、单唾液酸化Lea和双唾液酸化Lea；2型链：N-乙酰乳糖胺（血型H抗原的直接前体）、分支N-乙酰乳糖胺、H抗原、Lex和Ley；3型链：H抗原。T抗原表达良好，而Tn抗原则不然。正如预期的那样，在24例中有10例发现了A抗原。与A相关的抗原：ALeb、ALed和ALey仅在这10个标本中的少数几个中发现，而3型链A重复序列在所有标本中均有发现。由于msLea、Lex、Ley和T抗原已被描述为肿瘤相关抗原，我们得出结论，几种与肿瘤相关的碳水化合物抗原在胎儿胰腺中表达。
