Okada Y, Tsuji T
First Department of Internal Medicine, Okayama University Medical School, Japan.
J Immunol Methods. 1988 Sep 13;112(2):243-9. doi: 10.1016/0022-1759(88)90364-x.
With the use of a panel of monoclonal antibodies against structurally related carbohydrate antigens, finely regulated cellular phenotype of the carbohydrate antigens can be characterized. As a model system, the expression of N-acetyllactosamine-related antigens in human biliary epithelial cells was investigated using 16 murine monoclonal antibodies against different carbohydrate epitopes. Human biliary epithelial cells expressed type 1 backbone-related antigens at all synthetic stages. Type 2 backbone and its monofucosylated structures, i.e., Lex and H, were never detected. More complex type 2 chain antigens, i.e., Ley, A and ALey, were apparently expressed. Types 3 and 4 chain antigens were also shown to be expressed. However, the expression of N-acetyllactosamine-related antigen was polymorphic and differed from one individual to another. Furthermore, in spite of the topographical continuity of the biliary tree, biliary epithelial cells were not always equal in the expression of N-acetyllactosamine-related antigens. As a conclusion, not only the localization but also the structure of carbohydrate antigens can be immunohistochemically studied on the tissue sections with the use of well defined monoclonal antibodies.
通过使用一组针对结构相关碳水化合物抗原的单克隆抗体，可以对碳水化合物抗原的精细调节细胞表型进行表征。作为一个模型系统，使用16种针对不同碳水化合物表位的鼠单克隆抗体研究了人胆管上皮细胞中与N-乙酰乳糖胺相关抗原的表达。人胆管上皮细胞在所有合成阶段均表达与1型主链相关的抗原。从未检测到2型主链及其单岩藻糖基化结构，即Lex和H。明显表达了更复杂的2型链抗原，即Ley、A和ALey。还显示出3型和4型链抗原的表达。然而，N-乙酰乳糖胺相关抗原的表达具有多态性，个体之间存在差异。此外，尽管胆管树在地形上具有连续性，但胆管上皮细胞在N-乙酰乳糖胺相关抗原的表达上并不总是相同的。总之，使用定义明确的单克隆抗体，可以在组织切片上通过免疫组织化学方法研究碳水化合物抗原的定位和结构。
