Department of Genetics and Genetic Engineering, Faculty of Agriculture, Benha University, Benha, Egypt.
Department of Physiology, National Organization for Drug Control and Research (NODCAR), Giza, Egypt.
J Appl Microbiol. 2019 Apr;126(4):1278-1289. doi: 10.1111/jam.14200. Epub 2019 Feb 18.
Cyanobacteria are immense sources of several pharmacological active compounds such as flavonoids and carotenoids with anti-inflammatory and antioxidant activity. The potential therapeutic effect of two novel cyanobacterial isolates, Cronbergia siamensis (KY296358.1) and Sphaerospermopsis aphanizomenoides (KU212886.1), against hydrogen peroxide (H O )-induced oxidative stress damage in the rat model was determined in this study.
In vitro antioxidant activity of the two studied isolates was evaluated by radical scavenging assay and ferric reducing power. The possible prophylactic activity of S. aphanizomenoides (KU212886.1) against H O -induced oxidative stress in the rat model was assessed in vivo. Serum alanine transaminase and aspartate transaminase were measured for the liver functions in redox rats. Liver malondialdehyde (MDA), glutathione, oxidized glutathione, nitric oxide, superoxide dismutase (SOD) and catalase (CAT) were assessed as oxidative stress markers. The effect of S. aphanizomenoides on the transcripts level of superoxide dismutase (Mn-SOD) and catalase (CAT) genes in the rat's liver tissues was measured using qRT-PCR. Oral administration of S. aphanizomenoides extract in low and high doses (100, 200 mg kg b.w) resulted in significant improvement in biochemical parameters of liver functions and oxidative stress markers. Also, the endogenous antioxidant defence enzymes and the expression of their related genes (Mn/SOD, CAT) were upregulated. Immunohistochemistry of Caspase-3, an apoptotic marker, showed potent amelioration in the liver tissues.
The novel isolate S. aphanizomenoides proved in vitro and in vivo antioxidant activity against redox rat model.
This isolate provides a new source of pharmacological compounds with great importance in pharmacological and medical fields.
蓝藻是多种具有抗炎和抗氧化活性的药理学活性化合物（如类黄酮和类胡萝卜素）的巨大来源。本研究旨在确定两种新型蓝藻分离物 Cronbergia siamensis（KY296358.1）和 Sphaerospermopsis aphanizomenoides（KU212886.1）对过氧化氢（H₂O₂）诱导的氧化应激损伤在大鼠模型中的潜在治疗作用。
通过自由基清除测定法和铁还原能力评估了两种研究分离物的体外抗氧化活性。在体内评估了 S. aphanizomenoides（KU212886.1）对 H₂O₂诱导的氧化应激的可能预防作用。在氧化还原大鼠中测量血清丙氨酸转氨酶和天冬氨酸转氨酶以评估肝功能。评估肝丙二醛（MDA）、谷胱甘肽、氧化型谷胱甘肽、一氧化氮、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）作为氧化应激标志物。使用 qRT-PCR 测量 S. aphanizomenoides 对大鼠肝组织中超氧化物歧化酶（Mn-SOD）和过氧化氢酶（CAT）基因转录水平的影响。S. aphanizomenoides 提取物的低剂量（100mg/kg b.w）和高剂量（200mg/kg b.w）口服给药可显著改善肝功能和氧化应激标志物的生化参数。此外，内源性抗氧化防御酶及其相关基因（Mn/SOD、CAT）的表达上调。凋亡标志物 Caspase-3 的免疫组织化学显示肝脏组织有明显改善。
新型分离物 S. aphanizomenoides 已证明具有针对氧化还原大鼠模型的体外和体内抗氧化活性。
这种分离物为药理学和医学领域提供了具有重要意义的新的药理学化合物来源。
