Guerreiro Adriana, Andrade Mariana A, Menezes Carina, Vilarinho Fernanda, Dias Elsa
Laboratory of Biology and Ecotoxicology, Department Environmental Health, National Institute of Health Dr. Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisboa, Portugal.
Faculty of Sciences, University of Lisbon, Campo Grande, 1749-016 Lisboa, Portugal.
Toxins (Basel). 2020 Aug 26;12(9):548. doi: 10.3390/toxins12090548.
Antioxidant compounds from cyanobacteria may constitute a natural alternative to current synthetic antioxidants, which contain preservatives and suspected toxicity. In this work, we evaluate the antioxidant potential of cyanobacterial strains of distinct species/genus isolated from freshwater (n = 6), soil (n = 1) and wastewater (n = 1) environments. Lyophilized biomass obtained from in-vitro cultures of those strains was extracted with ethanol and methanol. The antioxidant potential was evaluated by chemical (DPPH scavenging method, β-carotene bleaching assay, determination of total phenolic and total flavonoid compounds) and biological (HO-exposed HEK293T cell line model) approach. Some strains showed high yields of antioxidant activity by the DPPH assay (up to 10.7% IP/20.7 TE μg/mL) and by the β-carotene bleaching assay (up to 828.94 AAC), as well as significant content in phenolic (123.16 mg EAG/g DW) and flavonoid (900.60 mg EQR/g DW) compounds. Normalization of data in a "per cell" or "per cell volume" base might facilitate the comparison between strains. Additionally, most of the cyanobacterial extracts conferred some degree of protection to HEK293T cells against the HO-induced cytotoxicity. Freshwater (LMECYA 009) and (LMECYA 088), terrestrial (LMECYA 291) and wastewater (LEGE 06224) seem to be promising strains for further investigation on cyanobacteria antioxidant potential.
蓝藻中的抗氧化化合物可能构成当前合成抗氧化剂的天然替代品，后者含有防腐剂且存在潜在毒性。在本研究中，我们评估了从淡水（n = 6）、土壤（n = 1）和废水（n = 1）环境中分离出的不同物种/属的蓝藻菌株的抗氧化潜力。从这些菌株的体外培养物中获得的冻干生物质用乙醇和甲醇进行提取。通过化学方法（DPPH清除法、β-胡萝卜素漂白试验、总酚和总黄酮化合物的测定）和生物学方法（HO暴露的HEK293T细胞系模型）评估抗氧化潜力。一些菌株通过DPPH试验（高达10.7% IP/20.7 TE μg/mL）和β-胡萝卜素漂白试验（高达828.94 AAC）显示出高抗氧化活性产量，以及酚类（123.16 mg EAG/g DW）和黄酮类（900.60 mg EQR/g DW）化合物的显著含量。以“每细胞”或“每细胞体积”为基础对数据进行归一化处理可能有助于菌株之间的比较。此外，大多数蓝藻提取物对HEK293T细胞免受HO诱导的细胞毒性提供了一定程度的保护。淡水（LMECYA 009）和（LMECYA 088）、陆地（LMECYA 291）和废水（LEGE 06224）似乎是进一步研究蓝藻抗氧化潜力的有前景的菌株。
