University of Tunis El Manar, Center of Biotechnology of Borj Cedria, LR15CBBC05 Olive Biotechnology, 2050, Tunis, Tunisia.
University of Tunis El Manar, Center of Biotechnology of Borj Cedria, LR15CBBC03 Bioactive Substances, 2050, Tunis, Tunisia.
Microb Pathog. 2019 Mar;128:245-249. doi: 10.1016/j.micpath.2019.01.012. Epub 2019 Jan 8.
(1) Many studies demonstrated that olive oil and the leaves have several biological activities, but the works on olive twigs remain very limited. In this report, we investigated for anti-coxsackievirus B-3 (CVB-3) and anti-herpes virus type 2 (HSV-2) activities of olive twigs from two Tunisian varieties: Chemlali (CM) and Chétoui (CT). Extraction from the olive twigs was carried out using successively hexane, dichloromethane, ethyl acetate and methanol. The obtained extracts were evaluated for their anti-CVB-3 and anti-HSV-2 activities by MTT and plaque reduction assays, respectively. Only hexane extracts exhibited significant activity with a selectivity index of 6.32±0.67 and 5.24±0.82 for CM and CT, respectively. The active compound was isolated through bio-guided assays using Thin Layer Chromatography (TLC) and identified as 2,4-di-tert-butylphenol (2,4-DTBP) by gas chromatography coupled with mass spectrometry (GC-MS). This work is the first study that demonstrated an antiviral activity of both olive twigs and DTBP.
(1) 许多研究表明，橄榄油和橄榄叶具有多种生物活性，但对橄榄枝的研究仍然非常有限。在本报告中，我们研究了来自突尼斯两个品种 Chemlali（CM）和 Chétoui（CT）的橄榄枝的抗柯萨奇病毒 B-3（CVB-3）和抗单纯疱疹病毒 2 型（HSV-2）活性。橄榄枝的提取采用正己烷、二氯甲烷、乙酸乙酯和甲醇依次进行。通过 MTT 和蚀斑减少测定法分别评估所得提取物对 CVB-3 和 HSV-2 的活性。只有正己烷提取物表现出显著的活性，对 CM 和 CT 的选择性指数分别为 6.32±0.67 和 5.24±0.82。通过使用薄层色谱（TLC）进行生物导向测定，分离出活性化合物，并通过气相色谱-质谱联用（GC-MS）鉴定为 2,4-二叔丁基苯酚（2,4-DTBP）。这项工作是首次证明橄榄枝和 DTBP 具有抗病毒活性的研究。
