Department of Animal Nutrition, Faculty of Veterinary Medicine, Firat University, Elazig, Turkey.
Scientific and Regulatory Affairs, Nutrition 21 LLC, Purchase, NY, USA.
Food Chem Toxicol. 2019 Mar;125:242-251. doi: 10.1016/j.fct.2019.01.005. Epub 2019 Jan 8.
The purpose of this study was to test the effects of arginine-silicate-inositol complex (ASI), compared to a combination of the individual ingredients (A+S+I) of the ASI, on inflammatory markers and joint health in a collagen-induced arthritis (CIA) rat model. A total of 28 Wistar rats were divided into four groups: (i) Control; (ii) Arthritic group, rats subjected to CIA induction by injection of bovine collagen type II (A); (iii) Arthritic group treated with equivalent doses of the separate components of the ASI complex (arginine hydrochloride, silicon, and inositol) (A+S+I); (iv) Arthritic group treated with the ASI complex. The ASI complex treatment showed improved inflammation scores and markers over the arthritic control and the A+S+I group. ASI group had also greater levels of serum and joint-tissue arginine and silicon than the A+S+I group. Joint tissue IL-6, NF-κB, COX-2, TNF-α, p38 MAPK, WISP-1, and β-Catenin levels were lower in the ASI group compared to the other groups (P < 0.05 for all). In conclusion, these results demonstrate that the ASI complex may be effective in reducing markers of inflammation associated with joint health and that the ASI complex is more effective than a combination of the individual ingredients.
本研究旨在测试精氨酸-硅酸-肌醇复合物 (ASI) 的作用，与 ASI 的各个成分（A+S+I）的组合相比，在胶原诱导性关节炎 (CIA) 大鼠模型中对炎症标志物和关节健康的影响。总共将 28 只 Wistar 大鼠分为四组：（i）对照组；（ii）关节炎组，通过注射牛 II 型胶原诱导 CIA（A）；（iii）关节炎组，给予 ASI 复合物的单独成分（盐酸精氨酸、硅和肌醇）的等效剂量（A+S+I）；（iv）关节炎组，给予 ASI 复合物治疗。ASI 复合物治疗在炎症评分和标志物方面均优于关节炎对照组和 A+S+I 组。与 A+S+I 组相比，ASI 组的血清和关节组织中的精氨酸和硅水平也更高。与其他组相比，ASI 组的关节组织中 IL-6、NF-κB、COX-2、TNF-α、p38 MAPK、WISP-1 和 β-连环蛋白水平较低（P<0.05）。总之，这些结果表明，ASI 复合物可能有效降低与关节健康相关的炎症标志物，并且 ASI 复合物比单独成分的组合更有效。
