Shackleton David, Gray Nicola, Ives Lesley, Malsom Simon, Vanhinsbergh Des
Key Forensic Services, 4 Penfold Drive, Wymondham, Norfolk, NR18 0WZ, UK.
Key Forensic Services, 4 Penfold Drive, Wymondham, Norfolk, NR18 0WZ, UK.
Forensic Sci Int. 2019 Feb;295:179-188. doi: 10.1016/j.forsciint.2018.12.015. Epub 2018 Dec 21.
The RapidHIT ID is a new instrument which has been designed specifically for non-technical users in non-laboratory environments, for example police custody suites or border control. The development studies presented here were performed using the AmpFℓSTR NGMSElect™ Express STR kit with single source reference samples. The system has been successfully optimised for extraction, PCR and electrophoresis. Once optimised studies on sensitivity, in terms of DNA input template, sample repeatability and instrument reproducibility have shown that the instrument is capable of producing robust profiles within an 85min, window, using mock reference buccal samples. This work has been carried out within the validation framework provided by the United Kingdom Forensic Science Regulator and is a key stage in the validation of the RapidHIT ID for use in custody suites within the UK criminal justice system.
RapidHIT ID是一种专门为非实验室环境中的非技术用户设计的新仪器，例如警方拘留室或边境管制场所。这里展示的开发研究是使用AmpFℓSTR NGMSElect™ Express STR试剂盒和单源参考样本进行的。该系统已成功针对提取、聚合酶链反应（PCR）和电泳进行了优化。一旦在灵敏度方面（就DNA输入模板、样本重复性和仪器再现性而言）进行了优化研究，结果表明该仪器能够在85分钟的时间范围内，使用模拟参考口腔样本生成可靠的图谱。这项工作是在英国法医学监管机构提供的验证框架内开展的，并且是RapidHIT ID在英国刑事司法系统拘留室使用的验证中的一个关键阶段。
