Salceda Susana, Barican Arnaldo, Buscaino Jacklyn, Goldman Bruce, Klevenberg Jim, Kuhn Melissa, Lehto Dennis, Lin Frank, Nguyen Phong, Park Charles, Pearson Francesca, Pittaro Rick, Salodkar Sayali, Schueren Robert, Smith Corey, Troup Charles, Tsou Dean, Vangbo Mattias, Wunderle Justus, King David
IntegenX Inc, 5720 Stoneridge Drive, Suite 300, Pleasanton, CA, 94588, USA.
IntegenX Inc, 5720 Stoneridge Drive, Suite 300, Pleasanton, CA, 94588, USA.
Forensic Sci Int Genet. 2017 May;28:21-34. doi: 10.1016/j.fsigen.2017.01.005. Epub 2017 Jan 12.
The RapidHIT ID is a fully automated sample-to-answer system for short tandem repeat (STR)-based human identification. The RapidHIT ID has been optimized for use in decentralized environments and processes presumed single source DNA samples, generating Combined DNA Index System (CODIS)-compatible DNA profiles in less than 90min. The system is easy to use, requiring less than one minute of hands-on time. Profiles are reviewed using centralized linking software, RapidLINK™ (IntegenX, Pleasanton, CA), a software tool designed to collate DNA profiles from single or multiple RapidHIT ID systems at different geographic locations. The RapidHIT ID has been designed to employ GlobalFiler Express and AmpFLSTR NGMSElect™, Thermo Fisher Scientific (Waltham, MA) STR chemistries. The Developmental Validation studies were performed using GlobalFiler Express with single source reference samples according to Scientific Working Group for DNA Analysis Methods guidelines. These results show that multiple RapidHIT ID systems networked with RapidLINK software form a highly reliable system for wide-scale deployment in locations such as police booking stations and border crossings enabling real-time testing of arrestees, potential human trafficking victims, and other instances where rapid turnaround is essential.
RapidHIT ID是一种基于短串联重复序列（STR）的全自动人身份识别样本到答案系统。RapidHIT ID已针对分散式环境进行了优化，可处理假定为单源的DNA样本，在不到90分钟的时间内生成符合联合DNA索引系统（CODIS）标准的DNA图谱。该系统易于使用，实际操作时间不到一分钟。使用集中式链接软件RapidLINK™（IntegenX公司，加利福尼亚州普莱森顿）对图谱进行审查，该软件工具旨在整理来自不同地理位置的单个或多个RapidHIT ID系统的DNA图谱。RapidHIT ID设计采用赛默飞世尔科技（马萨诸塞州沃尔瑟姆）的GlobalFiler Express和AmpFLSTR NGMSElect™ STR化学试剂盒。根据DNA分析方法科学工作组的指南，使用GlobalFiler Express和单源参考样本进行了开发验证研究。这些结果表明，多个与RapidLINK软件联网的RapidHIT ID系统形成了一个高度可靠的系统，可在警察登记站和边境口岸等地点进行大规模部署，能够对被捕者、潜在的人口贩运受害者以及其他需要快速周转的情况进行实时检测。
