Gezer P Gizem, Liu G Logan, Kokini Jozef L
Department of Food Science and Human Nutrition, University of Illinois Urbana-Champaign, 1304 W. Pennsylvania Ave, Urbana, USA.
Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, 1406 W. Green St, Urbana, USA; Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, 13 208 North Wright Street, Urbana, USA.
Talanta. 2016 Apr 1;150:224-32. doi: 10.1016/j.talanta.2015.12.034. Epub 2015 Dec 13.
Peanuts are among the most common food allergies, which may result in life-threatening reactions in certain people. For this reason, it is very important to monitor the presence of peanuts in the food system. Biosensors are an emerging way of detecting allergen proteins. In this research, we present a surface enhanced Raman spectroscopy (SERS) technique to detect the main allergen protein, Ara h1. The sensors were biodegradable and made out of a corn protein, zein. Nanophotonic structures on zein films consisted of gold coated pyramid structures. It was found that both detection and quantification was possible by using a statistical clustering technique principal component analysis (PCA). An optimization in data processing yielded the result that baseline correction and shorter data collection times were needed in order to successfully cluster data. The limit of detection was found to be 0.14 mg/ml. Furthermore, specificity of the sensor was provided by functionalizing the surface with monoclonal antibodies of Ara h1. Antibody functionalization, and Ara h1 capturing was tested and identified by also utilizing PCA analysis. As a proof-of-concept, this study showed that a biodegradable platform can be used in detection of a peanut allergen protein, Ara h1, using surface enhanced Raman spectroscopy.
花生是最常见的食物过敏原之一，在某些人身上可能会引发危及生命的反应。因此，监测食品系统中花生的存在非常重要。生物传感器是检测过敏原蛋白的一种新兴方法。在本研究中，我们提出了一种表面增强拉曼光谱（SERS）技术来检测主要过敏原蛋白Ara h1。这些传感器是可生物降解的，由玉米蛋白玉米醇溶蛋白制成。玉米醇溶蛋白膜上的纳米光子结构由金涂层金字塔结构组成。研究发现，使用统计聚类技术主成分分析（PCA）可以进行检测和定量。数据处理的优化结果表明，为了成功地对数据进行聚类，需要进行基线校正并缩短数据采集时间。检测限为0.14 mg/ml。此外，通过用Ara h1单克隆抗体对表面进行功能化，提供了传感器的特异性。抗体功能化以及Ara h1捕获也通过PCA分析进行了测试和鉴定。作为概念验证，本研究表明，可生物降解平台可用于利用表面增强拉曼光谱检测花生过敏原蛋白Ara h1。
