Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350108, China.
Sensors (Basel). 2019 Jan 9;19(2):228. doi: 10.3390/s19020228.
A novel turn-on fluorescence assay was developed for the rapid detection of glutathione (GSH) based on the inner-filter effect (IFE) and redox reaction. Molybdenum disulfide quantum dots (MoS₂ QDs), which have stable fluorescent properties, were synthesized with hydrothermal method. Manganese dioxide nanosheets (MnO₂ NSs) were prepared by exfoliating the bulk δ-MnO₂ material in bovine serum albumin (BSA) aqueous solution. The morphology structures of the prepared nanoparticles were characterized by transmission electron microscope (TEM). Studies have shown that the fluorescence of MoS₂ QDs could be quenched in the presence of MnO₂ NSs as a result of the IFE, and is recovered after the addition of GSH to dissolve the MnO₂ NSs. The fluorescence intensity showed a good linear relationship with the GSH concentration in the range 20⁻2500 μM, the limit of detection was 1.0 μM. The detection method was applied to the analysis of GSH in human serum samples. This simple, rapid, and cost-effective method has great potential in analyzing GSH and in disease diagnosis.
一种基于内滤效应（IFE）和氧化还原反应的新型谷胱甘肽（GSH）荧光检测法被开发出来。采用水热法合成了具有稳定荧光性能的二硫化钼量子点（MoS₂ QDs）。通过在牛血清白蛋白（BSA）水溶液中剥离块状δ-MnO₂ 材料，制备了二氧化锰纳米片（MnO₂ NSs）。通过透射电子显微镜（TEM）对所制备的纳米颗粒的形态结构进行了表征。研究表明，在 MnO₂ NSs 的存在下，MoS₂ QDs 的荧光会由于内滤效应而被猝灭，而加入 GSH 后会溶解 MnO₂ NSs，荧光会恢复。荧光强度与 GSH 浓度在 20⁻2500 μM 范围内呈良好的线性关系，检测限为 1.0 μM。该检测方法已应用于人血清样品中 GSH 的分析。这种简单、快速、经济有效的方法在分析 GSH 和疾病诊断方面具有很大的潜力。
