School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, PR China.
The College of Chemistry, Zhengzhou University, Zhengzhou 450001, PR China.
Int J Biol Macromol. 2022 Jan 15;195:237-245. doi: 10.1016/j.ijbiomac.2021.12.005. Epub 2021 Dec 9.
In present study, a novel glutathione functionalized MoS quantum dots (GSH-MoS QDs) was synthesized from sodium molybdate dehydrate and glutathione by using a one-pot hydrothermal method. After they were characterized, the influence of GSH-MoS QDs on amyloid aggregation of bovine serum albumin (BSA) was investigated by various analytical methods including thioflavin T fluorescence assay, circular dichroism and transmission electron microscope. Moreover, the effect of GSH-MoS QDs on cytotoxicity induced by BSA amyloid fibrils and cell penetration were evaluated by MTT assay and confocal fluorescence imaging, respectively. The results indicated that the GSH-MoS QDs not only had good water solubility, excellent biocompatibility and low cytotoxicity, but also could obviously inhibit the aggregation of BSA and depolymerize the formed BSA aggregates. The data obtained from this work demonstrated that the GSH-MoS QDs is expected to become a candidate drug for the treatment of amyloid-related diseases.
在本研究中，通过使用一锅水热法，从钼酸钠和谷胱甘肽合成了一种新型的谷胱甘肽功能化的 MoS 量子点 (GSH-MoS QDs)。对其进行表征后，通过多种分析方法，包括硫代黄素 T 荧光测定法、圆二色性和透射电子显微镜，研究了 GSH-MoS QDs 对牛血清白蛋白 (BSA) 淀粉样聚集的影响。此外，通过 MTT 测定法和共聚焦荧光成像分别评估了 GSH-MoS QDs 对 BSA 淀粉样纤维诱导的细胞毒性和细胞穿透的影响。结果表明，GSH-MoS QDs 不仅具有良好的水溶性、优异的生物相容性和低细胞毒性，而且还可以明显抑制 BSA 的聚集并解聚形成的 BSA 聚集体。这项工作得到的数据表明，GSH-MoS QDs 有望成为治疗与淀粉样蛋白相关疾病的候选药物。
