Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA), Facultad de Ciencias Exactas-UNLP, CONICET, CIC, Calle 47 y 116 - 1900, La Plata, Argentina.
Laboratory of Food Chemistry and Nutrition Science, Institute of Food Science and Technology, Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Chinese Academy of Agricultural Sciences, N°2 Yuan Ming Yuan West Road, Haidian District, Beijing, 100193, China.
Plant Foods Hum Nutr. 2019 Mar;74(1):107-114. doi: 10.1007/s11130-018-0708-8.
Antiproliferative effect of Amaranthus mantegazzianus proteins and peptides released after simulated gastrointestinal digestion (DH% 37.8 ± 3.8) was investigated on human colon cancer cell line HT-29. Inhibition of proliferation of HT-29 cells was exhibited after a 24 h treatment with different concentrations of amaranth protein isolate (API) and the peptides released after digestion (DGS), presenting IC values of 1.35 ± 0.12 and 0.30 ± 0.07 mg soluble protein/mL, respectively. Lactate dehydrogenase assay indicated that both samples caused the loss of membrane integrity and cell lysis over HT-29 cells, and DAPI fluorescence microscopies evidenced typical apoptotic features. Moreover, Annexin V-FITC flow cytometry showed a significant increase of early apoptotic and late apoptotic/necrotic HT-29 cells compared to untreated ones, and caspase-3 assay confirmed the apoptosis induction with a 43.0 ± 10.3 and 65.8 ± 12.7% increase of caspase-3 activity produced by a 2 mg/mL treatment of API and DGS, respectively. In conclusion, amaranth peptides successfully released after simulated gastrointestinal digestion would exert a potential antiproliferative activity over HT-29 tumor cells. This effect was linked to the induction of cell necrosis and apoptosis, supporting the idea of using amaranth proteins as a potential food alternative ingredient for functional foods.
经模拟胃肠道消化后释放的苋菜蛋白和肽对人结肠癌细胞 HT-29 的抗增殖作用进行了研究。用不同浓度的苋菜蛋白分离物 (API) 和消化后释放的肽 (DGS) 处理 HT-29 细胞 24 小时后，显示出对细胞增殖的抑制作用，其 IC 值分别为 1.35±0.12 和 0.30±0.07 mg 可溶性蛋白/mL。乳酸脱氢酶测定表明，两种样品均导致 HT-29 细胞的膜完整性丧失和细胞裂解，DAPI 荧光显微镜显示出典型的凋亡特征。此外，Annexin V-FITC 流式细胞术显示与未经处理的细胞相比，早期凋亡和晚期凋亡/坏死的 HT-29 细胞显著增加，caspase-3 测定证实了凋亡诱导，API 和 DGS 处理 2 mg/mL 时 caspase-3 活性分别增加了 43.0±10.3%和 65.8±12.7%。总之，经模拟胃肠道消化后成功释放的苋菜肽对 HT-29 肿瘤细胞具有潜在的抗增殖活性。这种作用与细胞坏死和凋亡的诱导有关，支持将苋菜蛋白用作功能性食品的潜在食品替代成分的想法。
