Orsini Delgado María C, Nardo Agustina, Pavlovic Marija, Rogniaux Hélène, Añón María C, Tironi Valeria A
Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA), UNLP-CONICET, 47 y 116, 1900 La Plata, Argentina.
Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Saenz Pena 182, B1876BXD Bernal, Argentina.
Food Chem. 2016 Apr 15;197 Pt B:1160-7. doi: 10.1016/j.foodchem.2015.11.092. Epub 2015 Nov 18.
The objective of the present work was to separate and identify antioxidant peptides from a simulated gastrointestinal digest (Id) from Amaranthus mantegazzianus proteins (I), which has previously been demonstrated to have this activity. I and Id were separated by preparative RP-HPLC. Fractions were evaluated by the ORAC method and the more active ones were analyzed by LC-MS/MS. Each fraction presented diverse peptides from different proteins, most of them from the 11S globulin. After grouping the peptides from 11S globulin according to their overlapping sequences, and based on previous information about structure-activity relationships, ten sequences were synthesized, in order to evaluate their antioxidant activity. Four peptides presented interesting activity: AWEEREQGSR>YLAGKPQQEH∼IYIEQGNGITGM∼TEVWDSNEQ. They exhibited some of the structural characteristics already known to demonstrate this activity, all of them containing at least one bulky aromatic residue. All belonged to little structured, internal or exposed regions of the acid subunit of the 11S globulin.
本研究的目的是从先前已证明具有抗氧化活性的尾穗苋蛋白质的模拟胃肠道消化物（Id）中分离并鉴定抗氧化肽。I和Id通过制备型反相高效液相色谱（RP-HPLC）进行分离。通过氧自由基吸收能力（ORAC）法对各馏分进行评估，并对活性较高的馏分进行液相色谱-串联质谱（LC-MS/MS）分析。每个馏分都呈现出来自不同蛋白质的多种肽段，其中大部分来自11S球蛋白。根据11S球蛋白肽段的重叠序列对其进行分组，并基于先前关于构效关系的信息，合成了十个序列，以评估它们的抗氧化活性。四种肽段表现出有趣的活性：AWEEREQGSR>YLAGKPQQEH∼IYIEQGNGITGM∼TEVWDSNEQ。它们展现出了一些已知具有该活性的结构特征，所有这些肽段都至少含有一个庞大的芳香族残基。它们均属于11S球蛋白酸性亚基结构松散的内部或暴露区域。
