Farshid Behzad, Lalwani Gaurav, Shir Mohammadi Meisam, Simonsen John, Sitharaman Balaji
Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York, 11794.
Department of Materials Science and Engineering, Stony Brook University, Stony Brook, New York, 11794.
J Biomed Mater Res B Appl Biomater. 2017 Feb;105(2):406-419. doi: 10.1002/jbm.b.33565. Epub 2015 Nov 3.
This study investigates the mechanical properties and in vitro cytotoxicity of one- and two-dimensional boron nitride nanomaterials-reinforced biodegradable polymeric nanocomposites. Poly(propylene fumarate) (PPF) nanocomposites were fabricated using crosslinking agent N-vinyl pyrrolidone and inorganic nanomaterials: boron nitride nanotubes (BNNTs) and boron nitride nanoplatelets (BNNPs) dispersed at 0.2 wt % in the polymeric matrix. The incorporation of BNNPs and BNNTs resulted in a ∼38 and ∼15% increase in compressive (Young's) modulus, and ∼31 and ∼6% increase in compressive yield strength compared to PPF control, respectively. The nanocomposites showed a time-dependent increased protein adsorption for collagen I protein. The cytotoxicity evaluation of aqueous BNNT and BNNP dispersions (at 1-100 μg/mL concentrations) using murine MC3T3 preosteoblast cells showed ∼73-99% viability. The cytotoxicity evaluation of media extracts of nanocomposites before crosslinking, after crosslinking, and upon degradation (using 1×-100× dilutions) showed dose-dependent cytotoxicity responses. Crosslinked nanocomposites showed excellent (∼79-100%) cell viability, cellular attachment (∼57-67%), and spreading similar to cells grown on the surface of tissue culture polystyrene control. The media extracts of degradation products showed a dose-dependent cytotoxicity. The favorable cytocompatibility results in combination with improved mechanical properties of BNNT and BNNP nanocomposites opens new avenues for further in vitro and in vivo safety and efficacy studies towards bone tissue engineering applications. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 406-419, 2017.
本研究调查了一维和二维氮化硼纳米材料增强的可生物降解聚合物纳米复合材料的力学性能和体外细胞毒性。使用交联剂N-乙烯基吡咯烷酮和无机纳米材料制备聚富马酸丙二醇酯（PPF）纳米复合材料：将氮化硼纳米管（BNNTs）和氮化硼纳米片（BNNPs）以0.2 wt%的比例分散在聚合物基体中。与PPF对照相比，BNNPs和BNNTs的加入分别使压缩（杨氏）模量提高了约38%和15%，压缩屈服强度提高了约31%和6%。纳米复合材料对I型胶原蛋白的蛋白质吸附呈现出随时间增加的趋势。使用小鼠MC3T3前成骨细胞对水性BNNT和BNNP分散体（浓度为1-100μg/mL）进行的细胞毒性评估显示，细胞活力约为73-99%。对纳米复合材料交联前、交联后以及降解时（使用1×-100×稀释度）的培养基提取物进行的细胞毒性评估显示出剂量依赖性的细胞毒性反应。交联后的纳米复合材料表现出优异的（约79-100%）细胞活力、细胞附着（约57-67%），并且细胞铺展情况与生长在组织培养聚苯乙烯对照表面的细胞相似。降解产物的培养基提取物表现出剂量依赖性的细胞毒性。BNNT和BNNP纳米复合材料良好的细胞相容性结果以及其改善的力学性能，为进一步开展骨组织工程应用的体外和体内安全性及有效性研究开辟了新途径。© 2015威利期刊公司。《生物医学材料研究杂志》B部分：应用生物材料，105B：406-419，2017。
