克隆并测序HinfI限制与修饰基因。
Cloning and sequencing the HinfI restriction and modification genes.
作者信息
Chandrasegaran S, Lunnen K D, Smith H O, Wilson G G
机构信息
Department of Environmental Health Sciences, Johns Hopkins University, School of Hygiene and Public Health, Baltimore, MD 21205.
出版信息
Gene. 1988 Oct 30;70(2):387-92. doi: 10.1016/0378-1119(88)90210-7.
The HinfI restriction and modification genes were cloned on a 3.9-kb PstI fragment inserted into the PstI site of plasmid pBR322. Both genes are confined to an internal 2.3-kb BclI-AvaI subfragment. This subfragment was sequenced. Two large open reading frames (ORF's) are present. ORF1 codes for the methylase [predicted 359 amino acids (aa)] and ORF2 codes for the endonuclease (predicted 262 or 272 aa).
HinfI 限制与修饰基因被克隆在一个插入质粒 pBR322 的 PstI 位点的 3.9-kb PstI 片段上。这两个基因都位于一个内部的 2.3-kb BclI-AvaI 亚片段中。对该亚片段进行了测序。存在两个大的开放阅读框（ORF）。ORF1 编码甲基化酶（预测有 359 个氨基酸（aa）），ORF2 编码内切核酸酶（预测有 262 或 272 个 aa）。
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