HhaI和HinPI限制修饰系统的克隆

Cloning of the HhaI and HinPI restriction-modification systems.

作者信息

Barsomian J M, Card C O, Wilson G G

机构信息

New England Biolabs, Inc., Beverly, MA 01915.

出版信息

Gene. 1988 Dec 25;74(1):5-7. doi: 10.1016/0378-1119(88)90237-5.

DOI:10.1016/0378-1119(88)90237-5

PMID:3074018

Abstract

The genes for the HhaI (Roberts et al., 1976) and HinPI (Roberts, 1987) restriction-modification (R-M) systems have been cloned in pBR322. The HhaI system was isolated on a 9-kb PstI fragment, and the HinPI system was isolated on two PstI fragments of 1.5 and 4.6 kb in length. The clones were isolated by selecting for recombinant molecules that had protectively modified themselves. The HhaI and HinPI R-M systems recognize the same sequence, GCGC, but hybridization between the DNA fragments encoding them does not take place.

摘要

HhaI（罗伯茨等人，1976年）和HinPI（罗伯茨，1987年）限制修饰（R-M）系统的基因已被克隆到pBR322中。HhaI系统是在一个9 kb的PstI片段上分离得到的，而HinPI系统是在两个长度分别为1.5 kb和4.6 kb的PstI片段上分离得到的。这些克隆是通过选择对自身进行了保护性修饰的重组分子而分离出来的。HhaI和HinPI R-M系统识别相同的序列GCGC，但编码它们的DNA片段之间不会发生杂交。