A new isoflavone with anti-inflammatory effect from the seeds of .

A new isoflavone, milletenol A (), along with four known flavonoids (-) were isolated from the seeds of . The structure of was established by extensive spectroscopic methods while known compounds were identified by comparisons with literature data. Compound and showed significant anti-inflammatory activities against nitric oxide production in LPS-induced RAW264.7 macrophages. The state of CuSO-stimulated inflammation was effectively alleviated by compound in zebrafish. However, no significant cytotoxicity against human breast cancer cells was observed among all isolates.