Antibacterial and Inhibitory Activities against Nitric Oxide Production of Coumaronochromones and Prenylated Isoflavones from .

A chemical investigation of leaf and root extracts of led to the isolation and structural elucidation of four new prenylated isoflavones, millexatins G-J (-), and three new coumaronochromones, millexatins K-M (-), along with 16 known compounds. The structures of the new compounds were determined on the basis of NMR and MS data. Compound is a rare isoflavone having a 2-hydroxyethyl moiety at C-8, whereas the structures of compounds - formally arise from a ring closure through HO-2' and C-2. The absolute configurations at the C-2 and C-3 positions of and were determined from their ECD spectra through comparison with those of previously reported compounds. Most of compounds were evaluated for their inhibitory effects against nitric oxide (NO) production on RAW264.7 macrophages and their antibacterial activities. Compounds and inhibited NO production with IC values of 8.5 and 14.3 μM, respectively. Compounds and showed antibacterial activity against various Gram-positive bacteria with MIC values ranging from 2 to 8 μg/mL.