Kovács P, Török O, Csaba G
Department of Biology, Semmelweis University of Medicine, Budapest, Hungary.
Histochemistry. 1988;90(3):241-4. doi: 10.1007/BF00492513.
Binding of fluorescein-isothiocyanate-(FITC)-labeled insulin was followed up in the function of time in Chang liver cells pretreated and not pretreated with insulin. The not pretreated cells showed patching, but no capping of the receptors during the period of study (60 min), whereas the insulin-pretreated cells showed indications of capping already after 10 min. Patching of the insulin receptors was particularly conspicuous at the sites of cell-cell contact (at the intercellular junctions). Supra-nuclear patching occurred earlier in the control cultures, and on it followed the fluorescence of the nuclear chromatin.
在未用胰岛素预处理和用胰岛素预处理的Chang肝细胞中，对异硫氰酸荧光素（FITC）标记的胰岛素的结合随时间进行了跟踪。在研究期间（60分钟），未预处理的细胞出现了成斑现象，但未出现受体的封帽现象，而胰岛素预处理的细胞在10分钟后就显示出封帽的迹象。胰岛素受体的成斑现象在细胞-细胞接触部位（细胞间连接）尤为明显。对照培养物中核上成斑现象出现得更早，随后是核染色质的荧光。
