肠道细菌是黑莓对小鼠产生益处所必需的。
Gut bacteria are required for the benefits of black raspberries in mice.
作者信息
Pan Pan, Oshima Kiyoko, Huang Yi-Wen, Yearsley Martha, Zhang Jianying, Arnold Mark, Yu Jianhua, Wang Li-Shu
机构信息
Department of Medicine, Division of Hematology and Oncology, Medical College of Wisconsin, Milwaukee, WI, USA.
Department of Pathology, Johns Hopkins University, Baltimore, MD, USA.
出版信息
J Berry Res. 2018;8(4):239-249. doi: 10.3233/JBR-180337. Epub 2018 Dec 20.
BACKGROUND
The gut microbiota plays a pivotal role in the development of inflammatory bowel disease and colorectal cancer.
OBJECTIVE
To determine whether the gut microbiota is required for the chemoprotective effects of black raspberries (BRBs) in mice.
METHODS
mice were given (a) a control diet for 8 weeks, or (b) the control diet for 4 weeks and then a 5% BRB diet for additional 4 weeks, or (c) the control diet and antibiotics for 4 weeks followed by the 5% BRB diet and antibiotics for the next 4 weeks. At the end of the study, all the mice were euthanized, and colonic and intestinal polyps were counted. mRNA expression levels of and were determined in colon and small intestine of these mice by quantitative real-time PCR.
RESULTS
5% BRBs significantly suppressed intestinal and colonic polyp development in the mice, whereas antibiotics significantly abolished BRBs' chemoprotective effects. BRBs decreased mRNA levels of and in colon, whereas significantly enhanced mRNA levels of and were observed in small intestine of BRB-treated mice fed antibiotics.
CONCLUSIONS
The gut microbiota is required for BRBs' chemoprotection against polyp development in mice.
背景
肠道微生物群在炎症性肠病和结直肠癌的发展中起关键作用。
目的
确定肠道微生物群对于黑莓（BRB）对小鼠的化学保护作用是否是必需的。
方法
将小鼠分为三组：（a）给予对照饮食8周；（b）给予对照饮食4周，然后给予5%黑莓饮食4周；（c）给予对照饮食和抗生素4周，随后给予5%黑莓饮食和抗生素4周。在研究结束时，对所有小鼠实施安乐死，并对结肠和肠道息肉进行计数。通过定量实时PCR测定这些小鼠结肠和小肠中相关基因的mRNA表达水平。
结果
5%的黑莓显著抑制了小鼠肠道和结肠息肉的发展，而抗生素显著消除了黑莓的化学保护作用。黑莓降低了结肠中相关基因的mRNA水平，而在喂食抗生素的黑莓处理小鼠的小肠中观察到相关基因的mRNA水平显著升高。
结论
肠道微生物群是黑莓对小鼠息肉发展进行化学保护所必需的。
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