Department of Chemistry, School of Physical and Mathematical Sciences, University of Kerala, Kariavattom, Trivandrum, Kerala, 695581, India.
Anal Bioanal Chem. 2019 Feb;411(5):997-1007. doi: 10.1007/s00216-018-1511-y. Epub 2019 Jan 14.
A fluorescent sensing platform using KI-quenched bovine serum albumin stabilized gold nanoclusters has been designed and used as a fluorescent probe for the turn-on detection of homocysteine/cysteine (Cys/Hcy). The fluorescence of gold nanoclusters was quenched by iodine. The fluorescence of quenched gold nanoclusters was effectively switched on by Cys/Hcy devoid of the interference of glutathione. The transmission electron microscopy image, X-ray photoelectron spectroscopy analysis, time-correlated single photon counting analysis, and dynamic light scattering data confirmed the aggregation-induced quenching of fluorescence of gold nanoclusters by iodine. The turn-on response of Cys/Hcy shows two linear ranges from 0.0057 to 5 μM and from 8 to 25 μM, with a limit of detection of 9 nM for cysteine and 12 nM for homocysteine. Real samples were analyzed to monitor Cys/Hcy added to human serum. The fluorescence turn-on response of the probe on a paper strip in the presence of Cys/Hcy was studied. Graphical abstract ᅟ.
设计了一种基于 KI 猝灭牛血清白蛋白稳定的金纳米簇的荧光传感平台，将其用作用于检测同型半胱氨酸/半胱氨酸（Cys/Hcy）的荧光探针。金纳米簇的荧光被碘猝灭。金纳米簇的荧光通过 Cys/Hcy 有效地打开，而不受谷胱甘肽的干扰。透射电子显微镜图像、X 射线光电子能谱分析、时间相关单光子计数分析和动态光散射数据证实了碘对金纳米簇荧光的聚集诱导猝灭。Cys/Hcy 的开启响应显示出两个线性范围，从 0.0057 到 5 μM 和从 8 到 25 μM，半胱氨酸的检测限为 9 nM，同型半胱氨酸的检测限为 12 nM。分析实际样品以监测添加到人血清中的 Cys/Hcy。研究了在 Cys/Hcy 存在下纸条上探针的荧光开启响应。示意图。
