U.S. Department of Agriculture, Agricultural Research Service, Southern Regional Research Center, 1100 Robert E. Lee Blvd, New Orleans, LA, 70124, USA.
Modern Research Center for Traditional Chinese Medicine and Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, China.
Plant Foods Hum Nutr. 2019 Mar;74(1):115-121. doi: 10.1007/s11130-018-0709-7.
Cinnamon polyphenol extract (CPE) improves people with insulin resistance. The objective was to investigate CPE and insulin on diacylglycerol acyltransferase (DGAT) gene expression important for lipid biosynthesis and compared it to anti-inflammatory tristetraprolin/zinc finger protein 36 (TTP/ZFP36) gene expression known to be regulated by both agents. Mouse 3T3-L1 adipocytes and RAW264.7 macrophages were treated with insulin and CPE followed by qPCR evaluation of DGAT and TTP mRNA levels. Insulin decreased DGAT1 and DGAT2 mRNA levels in adipocytes but had no effect on DGAT1 and increased DGAT2 mRNA levels 3-fold in macrophages. Insulin increased TTP mRNA levels 3-fold in adipocytes but had no effect in macrophages. CPE effect on DGAT1 gene expression was minimal but increased DGAT2 mRNA levels 2-4 fold in adipocytes and macrophages. CPE increased TTP mRNA levels 2-7 fold in adipocytes and macrophages. We conclude that CPE and insulin exhibited overlapping and independent effects on DGAT and TTP gene expression and suggest that CPE and insulin have profound effects on fat biosynthesis and inflammatory responses.
肉桂多酚提取物（CPE）可改善胰岛素抵抗的人群。目的是研究 CPE 和胰岛素对二酰基甘油酰基转移酶（DGAT）基因表达的影响，DGAT 基因表达对脂质生物合成很重要，同时也比较了其抗炎三肽重复蛋白（TTP）/锌指蛋白 36（ZFP36）基因表达的情况，已知这两种药物都可以调节 TTP/ZFP36 基因表达。用胰岛素和 CPE 处理小鼠 3T3-L1 脂肪细胞和 RAW264.7 巨噬细胞，然后通过 qPCR 评估 DGAT 和 TTP mRNA 水平。胰岛素降低脂肪细胞中 DGAT1 和 DGAT2 的 mRNA 水平，但对巨噬细胞中的 DGAT1 没有影响，而增加 DGAT2 的 mRNA 水平 3 倍。胰岛素使脂肪细胞中 TTP mRNA 水平增加 3 倍，但对巨噬细胞没有影响。CPE 对 DGAT1 基因表达的影响很小，但可使脂肪细胞和巨噬细胞中 DGAT2 的 mRNA 水平增加 2-4 倍。CPE 使脂肪细胞和巨噬细胞中 TTP mRNA 水平增加 2-7 倍。我们得出结论，CPE 和胰岛素对 DGAT 和 TTP 基因表达具有重叠和独立的作用，并表明 CPE 和胰岛素对脂肪生物合成和炎症反应有深远的影响。
