de Souza Gabriela Kalinowski, Sampaio Jamilla, Longoni Letícia, Ferreira Silviane, Alvarenga Samuel, Beneduzi Anelise
Universidade La Salle, Av. Vítor Barreto, 2288, Canoas, RS, CEP 92010-000, Brazil.
Departamento de Diagnóstico e Pesquisa Agropecuária (ex-FEPAGRO) da Secretaria da Agricultura, Pecuária e Irrigação (SEAPI) do Rio Grande do Sul, Rua Gonçalves Dias, 570, Porto Alegre, RS, CEP 90130-060, Brazil.
Braz J Microbiol. 2019 Jan;50(1):205-211. doi: 10.1007/s42770-018-0028-z. Epub 2018 Nov 29.
The bacterial strains SEMIA 587 and 5019 (Bradyrhizobium elkanii), 5079 (Bradyrhizobium japonicum), and 5080 (Bradyrhizobium diazoefficiens) are recommended for soybean inoculants in Brazil. In several countries, the current regulations are insufficient to induce companies for improving the quality of their products, leading to low performance and subsequent abandonment of inoculant use. From 2010 to 2014, 1086 samples coming mainly from Argentina and the southern region of Brazil were analyzed for viable cells counting, strains identification, and purity analysis according to the SDA/MAPA no. 30/2010 Normative Instruction. Most products were imported and formulated in liquid carriers with 5.0 × 10 colony-forming units (CFU)/mL. The strains most frequently used were SEMIA 5079/5080. Only 2.21% of samples had contaminants. The guaranteed concentration of viable cells in inoculants mostly ranged from 4.1 × 10 to 5.0 × 10 CFU/mL or CFU/g. The most frequently found concentration was above 1.1 × 10 CFU/mL or CFU/g, which was higher than the product guarantee. The inoculants used for soybean crop in Brazil have excellent quality, leading the country to the leadership in taking advantage of the biological nitrogen fixation benefits for a productive and sustainable agriculture.
巴西推荐使用SEMIA 587和5019菌株（埃尔坎根瘤菌）、5079菌株（日本慢生根瘤菌）以及5080菌株（高效固氮根瘤菌）制作大豆接种剂。在一些国家，现行法规不足以促使企业提高产品质量，导致接种剂性能不佳，随后接种剂的使用被弃用。2010年至2014年期间，根据第30/2010号规范性指令SDA/MAPA，对主要来自阿根廷和巴西南部地区的1086个样本进行了活细胞计数、菌株鉴定和纯度分析。大多数产品是进口的，且以液体载体配制，含有5.0×10个菌落形成单位（CFU）/mL。最常使用的菌株是SEMIA 5079/5080。只有2.21%的样本有污染物。接种剂中活细胞的保证浓度大多在4.1×10至5.0×10 CFU/mL或CFU/g之间。最常发现的浓度高于1.1×10 CFU/mL或CFU/g，高于产品保证值。巴西用于大豆作物的接种剂质量优良，使该国在利用生物固氮优势实现高产和可持续农业方面处于领先地位。
