利用解脂耶氏酵母生产油脂的整体方法。
Holistic Approaches in Lipid Production by Yarrowia lipolytica.
机构信息
Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA; Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 37, 51-630 Wroclaw, Poland; These authors contributed equally.
Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA; These authors contributed equally.
出版信息
Trends Biotechnol. 2018 Nov;36(11):1157-1170. doi: 10.1016/j.tibtech.2018.06.007. Epub 2018 Jul 11.
Concerns about climate change have driven research on the production of lipid-derived biofuels as an alternative and renewable liquid fuel source. Using oleaginous yeasts for lipid synthesis creates the potential for cost-effective industrial-scale operations due to their ability to reach high lipid titer, yield, and productivity resulting from their unique metabolism. Yarrowia lipolytica is the model oleaginous yeast, with the best-studied lipid metabolism, the greatest number of genetic tools, and a fully sequenced genome. In this review we highlight multiomics studies that elucidate the mechanisms allowing this yeast to achieve lipid overaccumulation and then present several major metabolic engineering efforts that enhanced the production metrics in Y. lipolytica. Recent achievements that applied novel engineering strategies are emphasized.
人们对气候变化的担忧推动了脂质衍生生物燃料生产的研究，将其作为一种替代的可再生液体燃料来源。利用产油酵母进行脂质合成具有潜在的成本效益，因为它们能够达到高脂质滴度、产率和生产力，这是由于它们独特的代谢方式。解脂耶氏酵母是模式产油酵母，其脂质代谢研究最为透彻，遗传工具最多，基因组也已完全测序。在这篇综述中，我们重点介绍了阐明使该酵母实现脂质过量积累的机制的多组学研究，然后介绍了几项旨在提高解脂耶氏酵母生产指标的主要代谢工程努力。强调了最近应用新型工程策略所取得的成就。
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