Oliveira Júnior Carlos Augusto, Silva Rodrigo Otávio Silveira, Cruz Diogo Soares Gonçalves, Pires Isadora Honorato, Alves Guilherme Guerra, Lobato Francisco Carlos Faria
Veterinary School, Federal University of Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG, CEP 31270-901, Brazil.
Braz J Microbiol. 2019 Jan;50(1):263-269. doi: 10.1007/s42770-018-0023-4. Epub 2018 Dec 18.
The oral administration of non-toxigenic strains of Clostridioides difficile (NTCD) is currently showing promising results for the prevention of Clostridioides difficile infection (CDI) in humans and animals, and is being considered as a possible commercial product to be used in the near future. The aim of this work was to evaluate five culture media for the growth and sporulation of one NTCD (Z31) and evaluate the viability of a lyophilized spore solution of NTCD Z31 stored at 4 °C or at 25 °C for 2 years. Reinforced clostridial medium (RCM) and brain heart infusion broth (BHI) provided the highest production of NTCD Z31 spores. In the first 6 months of the storage of the lyophilized solution, a reduction in spore count of approximately 0.3 Log CFU/mL was observed; however, no further significant reduction in spore count was observed up to 24 months. No difference in spore concentration was found between the two storage temperatures from 6 to 24 months of storage. The present work showed BHI and RCM to be the best choices for the growth and sporulation of NTCD Z31 and suggested that the spores of NTCD Z31 are stable for up to 2 years under both temperature conditions.
口服无毒力艰难梭菌（NTCD）菌株目前在预防人类和动物艰难梭菌感染（CDI）方面显示出有前景的结果，并被视为在不久的将来可能使用的商业产品。这项工作的目的是评估五种培养基对一种NTCD（Z31）生长和产孢的影响，并评估在4°C或25°C下储存2年的NTCD Z31冻干孢子溶液的活力。强化梭菌培养基（RCM）和脑心浸液肉汤（BHI）产生的NTCD Z31孢子最多。在冻干溶液储存的前6个月，观察到孢子计数减少约0.3 Log CFU/mL；然而，直到24个月，孢子计数没有进一步显著减少。在储存6至24个月期间，两种储存温度下的孢子浓度没有差异。目前的工作表明BHI和RCM是NTCD Z31生长和产孢的最佳选择，并表明NTCD Z31的孢子在两种温度条件下均可稳定保存2年。
